Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN_Sll0205-like-Phd
Location 3122397..3123001 Replicon chromosome
Accession NZ_CP089611
Organism Mycobacterium tuberculosis strain I0004240-3

Toxin (Protein)


Gene name vapC Uniprot ID P71623
Locus tag LVJ90_RS14875 Protein ID WP_003414492.1
Coordinates 3122397..3122789 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A829CBY8
Locus tag LVJ90_RS14880 Protein ID WP_003414495.1
Coordinates 3122786..3123001 (-) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ90_RS14845 (LVJ90_14850) 3117547..3118335 - 789 WP_003917092.1 CRISPR-associated endoribonuclease Cas6 -
LVJ90_RS14850 (LVJ90_14855) 3118669..3119214 - 546 WP_003913758.1 DUF1802 family protein -
LVJ90_RS14855 (LVJ90_14860) 3119486..3120370 - 885 WP_003414409.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
LVJ90_RS14860 (LVJ90_14865) 3120373..3121260 - 888 WP_003414414.1 type IV toxin-antitoxin system AbiEi family antitoxin -
LVJ90_RS14865 (LVJ90_14870) 3121565..3122110 - 546 WP_003899500.1 DUF1802 family protein -
LVJ90_RS14870 (LVJ90_14875) 3122107..3122376 - 270 WP_003912856.1 DUF2277 family protein -
LVJ90_RS14875 (LVJ90_14880) 3122397..3122789 - 393 WP_003414492.1 type II toxin-antitoxin system VapC family toxin Toxin
LVJ90_RS14880 (LVJ90_14885) 3122786..3123001 - 216 WP_003414495.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
LVJ90_RS14885 (LVJ90_14890) 3123048..3123797 + 750 WP_003414497.1 enoyl-CoA hydratase -
LVJ90_RS14890 (LVJ90_14895) 3123876..3124958 - 1083 WP_003414499.1 sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC -
LVJ90_RS14895 (LVJ90_14900) 3124951..3126261 - 1311 WP_003414501.1 ABC transporter substrate-binding protein -
LVJ90_RS14900 (LVJ90_14905) 3126264..3127091 - 828 WP_003414504.1 carbohydrate ABC transporter permease -
LVJ90_RS14905 (LVJ90_14910) 3127088..3127999 - 912 WP_003414505.1 sugar ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

VapC-like Sll0205 protein, PIN domain

(5-130)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(2-46)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14610.74 Da        Isoelectric Point: 6.7594

>T228554 WP_003414492.1 NZ_CP089611:c3122789-3122397 [Mycobacterium tuberculosis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Download         Length: 393 bp

>T228554 NZ_CP124322:976013-976420 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATTCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 7511.55 Da        Isoelectric Point: 4.9732

>AT228554 WP_003414495.1 NZ_CP089611:c3123001-3122786 [Mycobacterium tuberculosis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Download         Length: 216 bp

>AT228554 NZ_CP124322:975766-976032 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCACCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BWM8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CBY8

References