Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2856968..2857605 Replicon chromosome
Accession NZ_CP089611
Organism Mycobacterium tuberculosis strain I0004240-3

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag LVJ90_RS13410 Protein ID WP_003413180.1
Coordinates 2856968..2857363 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag LVJ90_RS13415 Protein ID WP_003413183.1
Coordinates 2857360..2857605 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ90_RS13370 (LVJ90_13370) 2852371..2853582 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
LVJ90_RS13375 (LVJ90_13375) 2853709..2854368 + 660 WP_003899369.1 LppA family lipoprotein -
LVJ90_RS13380 (LVJ90_13380) 2854365..2855027 + 663 WP_003899370.1 LppA family lipoprotein -
LVJ90_RS13385 (LVJ90_13385) 2855024..2855302 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
LVJ90_RS13390 (LVJ90_13390) 2855395..2855808 + 414 WP_003413164.1 PIN domain nuclease -
LVJ90_RS13395 (LVJ90_13395) 2855847..2856104 + 258 WP_003413167.1 CopG family transcriptional regulator -
LVJ90_RS13400 (LVJ90_13400) 2856101..2856478 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
LVJ90_RS13405 (LVJ90_13405) 2856494..2856868 - 375 WP_003413177.1 hypothetical protein -
LVJ90_RS13410 (LVJ90_13410) 2856968..2857363 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
LVJ90_RS13415 (LVJ90_13415) 2857360..2857605 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
LVJ90_RS13420 (LVJ90_13420) 2858016..2858435 - 420 WP_003413190.1 A24 family peptidase -
LVJ90_RS13425 (LVJ90_13425) 2858447..2859256 - 810 WP_003413193.1 shikimate dehydrogenase -
LVJ90_RS13430 (LVJ90_13430) 2859253..2860506 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
LVJ90_RS13435 (LVJ90_13435) 2860499..2861011 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T228547 WP_003413180.1 NZ_CP089611:c2857363-2856968 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T228547 NZ_CP124321:c115589-115284 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT228547 WP_003413183.1 NZ_CP089611:c2857605-2857360 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT228547 NZ_CP124321:c115809-115591 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References