Detailed information of TA system TA228544

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2841508..2842148
• Replicon: chromosome
• Accession: NZ_CP089611
• Organism: Mycobacterium tuberculosis strain I0004240-3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: LVJ90_RS13305
• Protein ID: WP_003412970.1
• Coordinates: 2841508..2841927 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: LVJ90_RS13310
• Protein ID: WP_003412975.1
• Coordinates: 2841924..2842148 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ90_RS13275 (LVJ90_13275)	2837093..2837815	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
LVJ90_RS13280 (LVJ90_13280)	2838332..2838559	+	228	WP_003412960.1	type II toxin-antitoxin system VapB family antitoxin	-
LVJ90_RS13285 (LVJ90_13285)	2838556..2838957	+	402	WP_003412963.1	PIN domain-containing protein	-
LVJ90_RS13290 (LVJ90_13290)	2838992..2839912	-	921	WP_003412965.1	restriction endonuclease	-
LVJ90_RS13295 (LVJ90_13295)	2840253..2840498	-	246	WP_071854223.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
LVJ90_RS13300 (LVJ90_13300)	2840557..2841507	+	951	WP_003911916.1	ERCC4 domain-containing protein	-
LVJ90_RS13305 (LVJ90_13305)	2841508..2841927	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ90_RS13310 (LVJ90_13310)	2841924..2842148	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
LVJ90_RS13315 (LVJ90_13315)	2842179..2845022	-	2844	WP_003899363.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
LVJ90_RS13320 (LVJ90_13320)	2845094..2845495	-	402	WP_003412981.1	hypothetical protein	-
LVJ90_RS13325 (LVJ90_13325)	2845495..2845965	-	471	WP_003899364.1	transcription antitermination factor NusB	-
LVJ90_RS13330 (LVJ90_13330)	2845968..2846531	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T228544 WP_003412970.1 NZ_CP089611:c2841927-2841508 [Mycobacterium tuberculosis]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T228544 NZ_CP124320:4252188-4252565 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCAACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAAAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT228544 WP_003412975.1 NZ_CP089611:c2842148-2841924 [Mycobacterium tuberculosis]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT228544 NZ_CP124320:4251731-4252111 [Escherichia coli]
ATGTCGGACACACTCCCCGGAACAACGCTTCCCGACGACAACAAAGACCGCCCCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGGGGCCGGT
TCAACAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGGTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCTGCGACCGAAACGGAAAGTAACCCTCCGGAATAG

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09