Detailed information of TA system TA228542

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/-
• Location: 2405462..2405991
• Replicon: chromosome
• Accession: NZ_CP089611
• Organism: Mycobacterium tuberculosis strain I0004240-3

Toxin (Protein)

• Gene name: parE
• Uniprot ID: G0TMR4
• Locus tag: LVJ90_RS11265
• Protein ID: WP_003411124.1
• Coordinates: 2405462..2405779 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: P9WJ74
• Locus tag: LVJ90_RS11270
• Protein ID: WP_003411127.1
• Coordinates: 2405776..2405991 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ90_RS11235 (LVJ90_11245)	2400483..2401559	+	1077	WP_003411110.1	hypothetical protein	-
LVJ90_RS11240 (LVJ90_11250)	2401556..2401837	+	282	WP_003411112.1	DUF5703 family protein	-
LVJ90_RS11245 (LVJ90_11255)	2401873..2402946	+	1074	WP_003411116.1	quinone-dependent dihydroorotate dehydrogenase	-
LVJ90_RS11250 (LVJ90_11260)	2402951..2403481	-	531	WP_003411119.1	YbhB/YbcL family Raf kinase inhibitor-like protein	-
LVJ90_RS11255 (LVJ90_11265)	2403529..2404875	-	1347	WP_003411121.1	M20/M25/M40 family metallo-hydrolase	-
LVJ90_RS11265 (LVJ90_11275)	2405462..2405779	-	318	WP_003411124.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LVJ90_RS11270 (LVJ90_11280)	2405776..2405991	-	216	WP_003411127.1	antitoxin ParD2	Antitoxin
LVJ90_RS11275 (LVJ90_11285)	2406246..2407304	+	1059	WP_003411129.1	phosphoribosyltransferase family protein	-
LVJ90_RS11280 (LVJ90_11290)	2407434..2407790	-	357	WP_003411130.1	hypothetical protein	-
LVJ90_RS11285 (LVJ90_11295)	2407885..2408667	-	783	WP_003411131.1	cell wall synthesis protein Wag31	-
LVJ90_RS11290 (LVJ90_11300)	2408935..2409225	-	291	WP_003900476.1	YggT family protein	-
LVJ90_RS11295 (LVJ90_11305)	2409387..2410043	-	657	WP_015456354.1	cell division protein SepF	-
LVJ90_RS11300 (LVJ90_11310)	2410109..2410885	-	777	WP_003411137.1	YggS family pyridoxal phosphate-dependent enzyme	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-92)

Antitoxin

• Conserved hypothetical protein CHP02574, addiction module (11-69)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12312.88 Da
• Isoelectric Point: 6.4831

>T228542 WP_003411124.1 NZ_CP089611:c2405779-2405462 [Mycobacterium tuberculosis]
MTRRLRVHNGVEDDLFEAFSYYADAAPDQIDRLYNLFVDAVTKRIPQAPNAFAPLFKHYRHIYLRPFRYYVAYRTTDEAI
DILAVRHGMENPNAVEAEISGRTFE

Nucleotide

• Length: 318 bp

>T228542 NZ_CP124320:c3214146-3213895 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7880.70 Da
• Isoelectric Point: 4.4055

>AT228542 WP_003411127.1 NZ_CP089611:c2405991-2405776 [Mycobacterium tuberculosis]
VVVNRALLASVDALSRDEQIELVEHINGNLAEGMHISEANQALIEARANDTDDAHWSTIDDFDKRIRARLG

Nucleotide

• Length: 216 bp

>AT228542 NZ_CP124320:c3213898-3213644 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: G0TMR4

Antitoxin

• AlphaFold DB: A0A7U4FAJ4