Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2235471..2236057 Replicon chromosome
Accession NZ_CP089611
Organism Mycobacterium tuberculosis strain I0004240-3

Toxin (Protein)


Gene name mazF Uniprot ID G0TLY3
Locus tag LVJ90_RS10465 Protein ID WP_003410010.1
Coordinates 2235471..2235815 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL58
Locus tag LVJ90_RS10470 Protein ID WP_003410014.1
Coordinates 2235809..2236057 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ90_RS10430 (LVJ90_10440) 2231177..2231776 + 600 WP_003409989.1 L-lysine exporter -
LVJ90_RS10435 (LVJ90_10445) 2232192..2232620 + 429 WP_003409992.1 cellulose-binding protein -
LVJ90_RS10440 (LVJ90_10450) 2232846..2233385 + 540 WP_003900446.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
LVJ90_RS10445 (LVJ90_10455) 2233905..2234465 - 561 WP_003410001.1 RES family NAD+ phosphorylase -
LVJ90_RS10450 (LVJ90_10460) 2234462..2234803 - 342 WP_003410003.1 DUF2384 domain-containing protein -
LVJ90_RS10455 (LVJ90_10465) 2234889..2235146 + 258 WP_003410006.1 hypothetical protein -
LVJ90_RS10460 (LVJ90_10470) 2235047..2235382 - 336 WP_003410009.1 dehydrogenase -
LVJ90_RS10465 (LVJ90_10475) 2235471..2235815 - 345 WP_003410010.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 Toxin
LVJ90_RS10470 (LVJ90_10480) 2235809..2236057 - 249 WP_003410014.1 ribbon-helix-helix protein, CopG family Antitoxin
LVJ90_RS10475 (LVJ90_10485) 2236157..2238472 - 2316 WP_003899120.1 cation transporter ATPase CptG -
LVJ90_RS10480 (LVJ90_10490) 2238469..2238741 - 273 WP_003410017.1 DUF1490 family protein -
LVJ90_RS10485 (LVJ90_10495) 2238794..2239150 - 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -
LVJ90_RS10490 (LVJ90_10500) 2239307..2240074 + 768 WP_003900447.1 hemerythrin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-38)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12230.12 Da        Isoelectric Point: 9.8887

>T228537 WP_003410010.1 NZ_CP089611:c2235815-2235471 [Mycobacterium tuberculosis]
VVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPGNVFLPATTTRLPRDSVVNVTA
IVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL

Download         Length: 345 bp

>T228537 NZ_CP124320:c2414342-2414235 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACAACCTGGCGGCACCGATCCTGGCGGGGATTATTACCGCAGTGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9290.34 Da        Isoelectric Point: 4.3757

>AT228537 WP_003410014.1 NZ_CP089611:c2236057-2235809 [Mycobacterium tuberculosis]
MKTAISLPDETFDRVSRRASELGMSRSEFFTKAAQRYLHELDAQLLTGQIDRALESIHGTDEAEALAVANAYRVLETMDD
EW

Download         Length: 249 bp

>AT228537 NZ_CP124320:2414390-2414456 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5HK3
PDB 5HK0
PDB 5HKC

Antitoxin

Source ID Structure
PDB 7WJ0
PDB 7WNR
AlphaFold DB A0A7U4FAW9

References