Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mbcAT/RES-TIGR02293
Location 2233905..2234803 Replicon chromosome
Accession NZ_CP089611
Organism Mycobacterium tuberculosis strain I0004240-3

Toxin (Protein)


Gene name mbcT Uniprot ID P64908
Locus tag LVJ90_RS10445 Protein ID WP_003410001.1
Coordinates 2233905..2234465 (-) Length 187 a.a.

Antitoxin (Protein)


Gene name mbcA Uniprot ID P64910
Locus tag LVJ90_RS10450 Protein ID WP_003410003.1
Coordinates 2234462..2234803 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ90_RS10415 (LVJ90_10425) 2229074..2229727 - 654 WP_003409976.1 cutinase Cfp21 -
LVJ90_RS10420 (LVJ90_10430) 2229842..2230072 + 231 WP_003409980.1 DUF167 domain-containing protein -
LVJ90_RS10425 (LVJ90_10435) 2230157..2231068 - 912 WP_003409986.1 ArgP/LysG family DNA-binding transcriptional regulator -
LVJ90_RS10430 (LVJ90_10440) 2231177..2231776 + 600 WP_003409989.1 L-lysine exporter -
LVJ90_RS10435 (LVJ90_10445) 2232192..2232620 + 429 WP_003409992.1 cellulose-binding protein -
LVJ90_RS10440 (LVJ90_10450) 2232846..2233385 + 540 WP_003900446.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
LVJ90_RS10445 (LVJ90_10455) 2233905..2234465 - 561 WP_003410001.1 RES family NAD+ phosphorylase Toxin
LVJ90_RS10450 (LVJ90_10460) 2234462..2234803 - 342 WP_003410003.1 DUF2384 domain-containing protein Antitoxin
LVJ90_RS10455 (LVJ90_10465) 2234889..2235146 + 258 WP_003410006.1 hypothetical protein -
LVJ90_RS10460 (LVJ90_10470) 2235047..2235382 - 336 WP_003410009.1 dehydrogenase -
LVJ90_RS10465 (LVJ90_10475) 2235471..2235815 - 345 WP_003410010.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 -
LVJ90_RS10470 (LVJ90_10480) 2235809..2236057 - 249 WP_003410014.1 ribbon-helix-helix protein, CopG family -
LVJ90_RS10475 (LVJ90_10485) 2236157..2238472 - 2316 WP_003899120.1 cation transporter ATPase CptG -
LVJ90_RS10480 (LVJ90_10490) 2238469..2238741 - 273 WP_003410017.1 DUF1490 family protein -
LVJ90_RS10485 (LVJ90_10495) 2238794..2239150 - 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(25-148)

Antitoxin

Antitoxin Xre/MbcA/ParS-like, toxin-binding domain

(69-110)


Sequences

Toxin        


Download         Length: 187 a.a.        Molecular weight: 20248.70 Da        Isoelectric Point: 4.5091

>T228536 WP_003410001.1 NZ_CP089611:c2234465-2233905 [Mycobacterium tuberculosis]
VSDALDEGLVQRIDARGTIEWSETCYRYTGAHRDALSGEGARRFGGRWNPPLLFPAIYLADSAQACMVEVERAAQAASTT
AEKMLEAAYRLHTIDVTDLAVLDLTTPQAREAVGLENDDIYGDDWSGCQAVGHAAWFLHMQGVLVPAAGGVGLVVTAYEQ
RTRPGQLQLRQSVDLTPALYQELRAT

Download         Length: 561 bp

>T228536 NZ_CP124320:1591258-1591560 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 12488.33 Da        Isoelectric Point: 4.8359

>AT228536 WP_003410003.1 NZ_CP089611:c2234803-2234462 [Mycobacterium tuberculosis]
MGVNVLASTVSGAIERLGLTYEEVGDIVDASPRSVARWTAGQVVPQRLNKQRLIELAYVADALAEVLPRDQANVWMFSPN
RLLEHRKPADLVRDGEYQRVLALIDAMAEGVFV

Download         Length: 342 bp

>AT228536 NZ_CP124320:1591596-1591937 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FKG

Antitoxin

Source ID Structure
PDB 6FKG
AlphaFold DB A0A7U4FAE4

References