Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC(toxin)
Location 2226579..2227267 Replicon chromosome
Accession NZ_CP089611
Organism Mycobacterium tuberculosis strain I0004240-3

Toxin (Protein)


Gene name vapC Uniprot ID P9WF64
Locus tag LVJ90_RS10400 Protein ID WP_003899117.1
Coordinates 2226579..2226998 (-) Length 140 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ28
Locus tag LVJ90_RS10405 Protein ID WP_003409968.1
Coordinates 2227007..2227267 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ90_RS10380 (LVJ90_10390) 2222074..2222922 + 849 WP_003409942.1 class I SAM-dependent methyltransferase -
LVJ90_RS10385 (LVJ90_10395) 2222885..2224330 - 1446 WP_003899115.1 APC family permease -
LVJ90_RS10390 (LVJ90_10400) 2224509..2225195 - 687 WP_003409954.1 immunoprotective protein Mpt64 -
LVJ90_RS10395 (LVJ90_10405) 2225386..2226354 - 969 WP_003899116.1 class 1b ribonucleoside-diphosphate reductase subunit beta -
LVJ90_RS10400 (LVJ90_10410) 2226579..2226998 - 420 WP_003899117.1 type II toxin-antitoxin system VapC family toxin Toxin
LVJ90_RS10405 (LVJ90_10415) 2227007..2227267 - 261 WP_003409968.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LVJ90_RS10410 (LVJ90_10420) 2227410..2229086 + 1677 WP_003899118.1 PecA family PE domain-processing aspartic protease -
LVJ90_RS10415 (LVJ90_10425) 2229074..2229727 - 654 WP_003409976.1 cutinase Cfp21 -
LVJ90_RS10420 (LVJ90_10430) 2229842..2230072 + 231 WP_003409980.1 DUF167 domain-containing protein -
LVJ90_RS10425 (LVJ90_10435) 2230157..2231068 - 912 WP_003409986.1 ArgP/LysG family DNA-binding transcriptional regulator -
LVJ90_RS10430 (LVJ90_10440) 2231177..2231776 + 600 WP_003409989.1 L-lysine exporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-125)

Antitoxin

Antitoxin VapB-like

(2-81)


Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 14748.87 Da        Isoelectric Point: 7.9722

>T228535 WP_003899117.1 NZ_CP089611:c2226998-2226579 [Mycobacterium tuberculosis]
MIVDTSAVVALVQGERPHATLVAAALAGAHSPVMSAPTVAECLIVLTARHGPVARTIFERLRSEIGLSVSSFTAEHAAAT
QRAFLRYGKGRHRAALNFGDCMTYATAQLGHQPLLAVGNDFPQTDHEFRGVVGYWPGVA

Download         Length: 420 bp

>T228535 NZ_CP124320:c1562304-1562086 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCTGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9528.88 Da        Isoelectric Point: 5.1007

>AT228535 WP_003409968.1 NZ_CP089611:c2227267-2227007 [Mycobacterium tuberculosis]
VALNIKDPEVDRLAAELADRLHTSKTAAIRHALSAQLAFLESRAGDREAQLLDILRTEIWPLLADRSPITKLEREQILGY
DPATGV

Download         Length: 261 bp

>AT228535 NZ_CP124320:c1562704-1562330 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E7TLI0

Antitoxin

Source ID Structure
AlphaFold DB A0A829C483

References