Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 1940029..1940642 Replicon chromosome
Accession NZ_CP089611
Organism Mycobacterium tuberculosis strain I0004240-3

Toxin (Protein)


Gene name vapC Uniprot ID P9WFA2
Locus tag LVJ90_RS09065 Protein ID WP_003408465.1
Coordinates 1940029..1940418 (-) Length 130 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ52
Locus tag LVJ90_RS09070 Protein ID WP_003408469.1
Coordinates 1940415..1940642 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ90_RS09030 (LVJ90_09035) 1935658..1936572 + 915 WP_003898985.1 3-hydroxyacyl-CoA dehydrogenase family protein -
LVJ90_RS09035 (LVJ90_09040) 1936575..1937405 + 831 WP_003408448.1 cyclase family protein -
LVJ90_RS09040 (LVJ90_09045) 1937405..1937755 + 351 WP_003898986.1 cupin domain-containing protein -
LVJ90_RS09045 (LVJ90_09050) 1937808..1938626 + 819 WP_003408456.1 3-keto-5-aminohexanoate cleavage protein -
LVJ90_RS09050 (LVJ90_09055) 1938640..1939419 + 780 WP_003408460.1 IclR family transcriptional regulator -
LVJ90_RS09060 (LVJ90_09065) 1939850..1939981 + 132 Protein_1787 IS3 family transposase -
LVJ90_RS09065 (LVJ90_09070) 1940029..1940418 - 390 WP_003408465.1 type II toxin-antitoxin system VapC family toxin Toxin
LVJ90_RS09070 (LVJ90_09075) 1940415..1940642 - 228 WP_003408469.1 antitoxin Antitoxin
LVJ90_RS09075 (LVJ90_09080) 1940860..1942344 + 1485 WP_003408473.1 biotin carboxylase -
LVJ90_RS09080 (LVJ90_09085) 1942341..1943588 + 1248 WP_003408476.1 serine hydrolase -
LVJ90_RS09085 (LVJ90_09090) 1943631..1944050 - 420 WP_003408483.1 hypothetical protein -
LVJ90_RS09090 (LVJ90_09095) 1944040..1944750 - 711 WP_003408486.1 winged helix-turn-helix transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-116)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 130 a.a.        Molecular weight: 13817.98 Da        Isoelectric Point: 7.0612

>T228530 WP_003408465.1 NZ_CP089611:c1940418-1940029 [Mycobacterium tuberculosis]
VIVLDASAAVELMLTTPAGAAVARRLRGETVHAPAHFDVEVIGAIRQAVVRQLISDHEGLVVVVNFLSLPVRRWPLKPFT
QRAYQLRSTHTVADGAYVALAEGLGVPLITCDGRLAQSHGHNAEIELVA

Download         Length: 390 bp

>T228530 NZ_CP124320:c703722-703345 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACGTTAAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGTATTGCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACCGACCAGCCGGGATTC
AGCGCAGGAACTTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCACGCCGGGCAACAGGTCTGATGACCCGCAG
CAACTACAGAACGGTAAATAACATTACCCGGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8226.39 Da        Isoelectric Point: 4.3313

>AT228530 WP_003408469.1 NZ_CP089611:c1940642-1940415 [Mycobacterium tuberculosis]
MSAMVQIRNVPDELLHELKARAAAQRMSLSDFLLARLAEIAEEPALDDVLDRLAALPRRDLGASAAELVDEARSE

Download         Length: 228 bp

>AT228530 NZ_CP124320:c704180-703812 [Escherichia coli]
GTGTCTGACACACTTTCCGGGGCAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCAGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BUI9

Antitoxin

Source ID Structure
PDB 7E4J
AlphaFold DB A0A7U4FAN9

References