Detailed information of TA system TA228529

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1767063..1767676
• Replicon: chromosome
• Accession: NZ_CP089611
• Organism: Mycobacterium tuberculosis strain I0004240-3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64880
• Locus tag: LVJ90_RS08315
• Protein ID: WP_003407786.1
• Coordinates: 1767272..1767676 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0E8UJ46
• Locus tag: LVJ90_RS08310
• Protein ID: WP_009935474.1
• Coordinates: 1767063..1767266 (+)
• Length: 68 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ90_RS08280 (LVJ90_08285)	1762468..1762848	+	381	WP_003407768.1	fumarate reductase subunit C	-
LVJ90_RS08285 (LVJ90_08290)	1762845..1763222	+	378	WP_003407771.1	fumarate reductase subunit FrdD	-
LVJ90_RS08290 (LVJ90_08295)	1763290..1763898	+	609	WP_003407773.1	TetR/AcrR family transcriptional regulator	-
LVJ90_RS08295 (LVJ90_08300)	1764082..1765230	+	1149	Protein_1636	MMPL family transporter	-
LVJ90_RS08300 (LVJ90_08305)	1765240..1765686	+	447	WP_003407780.1	F420H(2)-dependent quinone reductase	-
LVJ90_RS08305 (LVJ90_08310)	1765721..1767010	+	1290	WP_003407781.1	threonine ammonia-lyase	-
LVJ90_RS08310 (LVJ90_08315)	1767063..1767266	+	204	WP_009935474.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LVJ90_RS08315 (LVJ90_08320)	1767272..1767676	+	405	WP_003407786.1	type II toxin-antitoxin system toxin ribonuclease VapC11	Toxin
LVJ90_RS08320 (LVJ90_08325)	1767693..1769435	-	1743	WP_003407788.1	malto-oligosyltrehalose trehalohydrolase	-
LVJ90_RS08325 (LVJ90_08330)	1769428..1771725	-	2298	WP_003407790.1	malto-oligosyltrehalose synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-44)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14650.76 Da
• Isoelectric Point: 5.6587

>T228529 WP_003407786.1 NZ_CP089611:1767272-1767676 [Mycobacterium tuberculosis]
MILIDTSAWVEYFRATGSIAAVEVRRLLSEEAARIAMCEPIAMEILSGALDDNTHTTLERLVNGLPSLNVDDAIDFRAAA
GIYRAARRAGETVRSINDCLIAALAIRHGARIVHRDADFDVIARITNLQAASFR

Nucleotide

• Length: 405 bp

>T228529 NZ_CP124320:359106-359633 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCATT
AAATCTCTTTCTGACGACACATCTGGTTCGTCAACATCGCAATAAAATTCTGCGAGCGTATATCCTGTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATCCGGCAGTTGTTTTGAACGAGCCGCACTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTAACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTTGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATCAGTCGCTGGGCTTTATCCCATTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCGACCAAATCTATAGAGCTACTTTTTACACCAGACGATTAA

Antitoxin

Protein

• Length: 68 a.a.
• Molecular weight: 7501.48 Da
• Isoelectric Point: 4.3951

>AT228529 WP_009935474.1 NZ_CP089611:1767063-1767266 [Mycobacterium tuberculosis]
MSRTNIDIDDELAAEVMRRFGLTTKRAAVDLALRRLVGSPLSREFLLGLEGVGWEGDLDDLRSDRPD

Nucleotide

• Length: 204 bp

>AT228529 NZ_CP124320:358833-359099 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATTGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGAGCTGCGGAAGTGATTGAACAGCATCGGCGGA
TGATTCTCACTGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Structures

Toxin

• PDB: 6A7V

Antitoxin

• AlphaFold DB: A0A0E8UJ46