Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1687205..1687821 Replicon chromosome
Accession NZ_CP089611
Organism Mycobacterium tuberculosis strain I0004240-3

Toxin (Protein)


Gene name mazF Uniprot ID G0TJ74
Locus tag LVJ90_RS07960 Protein ID WP_003407593.1
Coordinates 1687504..1687821 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TJ73
Locus tag LVJ90_RS07955 Protein ID WP_003900349.1
Coordinates 1687205..1687507 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ90_RS07945 (LVJ90_07945) 1683091..1684938 + 1848 WP_003407585.1 methylmalonyl-CoA mutase small subunit -
LVJ90_RS07950 (LVJ90_07950) 1684939..1687191 + 2253 WP_003407587.1 methylmalonyl-CoA mutase -
LVJ90_RS07955 (LVJ90_07955) 1687205..1687507 + 303 WP_003900349.1 type II toxin-antitoxin system antitoxin MazE4 Antitoxin
LVJ90_RS07960 (LVJ90_07960) 1687504..1687821 + 318 WP_003407593.1 type II toxin-antitoxin system toxin endoribonuclease MazF4 Toxin
LVJ90_RS07965 (LVJ90_07965) 1687818..1688822 + 1005 WP_003407596.1 methylmalonyl Co-A mutase-associated GTPase MeaB -
LVJ90_RS07970 (LVJ90_07970) 1688875..1690164 + 1290 WP_015345075.1 serine hydrolase domain-containing protein -
LVJ90_RS07975 (LVJ90_07975) 1690237..1690962 - 726 WP_003898898.1 class I SAM-dependent methyltransferase -
LVJ90_RS07980 (LVJ90_07980) 1691068..1691280 - 213 WP_003898900.1 dodecin family protein -
LVJ90_RS07985 (LVJ90_07985) 1691341..1691739 + 399 WP_003900351.1 hypothetical protein -
LVJ90_RS07990 (LVJ90_07990) 1691784..1692812 + 1029 WP_003407612.1 glycosyltransferase family 2 protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-99)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 11377.97 Da        Isoelectric Point: 7.9950

>T228528 WP_003407593.1 NZ_CP089611:1687504-1687821 [Mycobacterium tuberculosis]
VNAPLRGQVYRCDLGYGAKPWLIVSNNARNRHTADVVAVRLTTTRRTIPTWVAMGPSDPLTGYVNADNIETLGKDELGDY
LGEVTPATMNKINTALATALGLPWP

Download         Length: 318 bp

>T228528 NZ_CP124320:256595-256702 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGTTCCGGTCATTACTGGCATTCTTGCCAGTATGAT
CGTGAGCTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11173.42 Da        Isoelectric Point: 9.5564

>AT228528 WP_003900349.1 NZ_CP089611:1687205-1687507 [Mycobacterium tuberculosis]
VPFLVALSGIISGVRDHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDEQLDAAYAAAIHDNPAYPYES
EAERSAARARRNARQQRSAQ

Download         Length: 303 bp

>AT228528 NZ_CP124320:c256538-256481 [Escherichia coli]
TCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5XE3
PDB 5XE2

Antitoxin

Source ID Structure
PDB 5XE3
AlphaFold DB G0TJ73

References