Detailed information of TA system TA228525

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-Phd
• Location: 1393747..1394306
• Replicon: chromosome
• Accession: NZ_CP089611
• Organism: Mycobacterium tuberculosis strain I0004240-3

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G0THS1
• Locus tag: LVJ90_RS06670
• Protein ID: WP_003898789.1
• Coordinates: 1393747..1394040 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G0THS2
• Locus tag: LVJ90_RS06675
• Protein ID: WP_003406322.1
• Coordinates: 1394037..1394306 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ90_RS06645 (LVJ90_06635)	1389341..1389601	+	261	WP_003406302.1	type II toxin-antitoxin system antitoxin VapB33	-
LVJ90_RS06650 (LVJ90_06640)	1389598..1390029	+	432	WP_003406304.1	type II toxin-antitoxin system VapC family toxin	-
LVJ90_RS06655 (LVJ90_06645)	1390052..1391739	-	1688	Protein_1313	PE family protein	-
LVJ90_RS06660 (LVJ90_06650)	1391919..1392779	+	861	WP_003900301.1	glycine betaine ABC transporter substrate-binding protein	-
LVJ90_RS06665 (LVJ90_06655)	1392860..1393690	-	831	WP_003898788.1	SDR family NAD(P)-dependent oxidoreductase	-
LVJ90_RS06670 (LVJ90_06660)	1393747..1394040	-	294	WP_003898789.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
LVJ90_RS06675 (LVJ90_06665)	1394037..1394306	-	270	WP_003406322.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LVJ90_RS06680 (LVJ90_06670)	1394419..1398114	-	3696	WP_003898790.1	multifunctional oxoglutarate decarboxylase/oxoglutarate dehydrogenase thiamine pyrophosphate-binding subunit/dihydrolipoyllysine-residue succinyltransferase subunit	-
LVJ90_RS06685 (LVJ90_06675)	1398256..1399044	-	789	WP_003900303.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (9-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11031.57 Da
• Isoelectric Point: 9.6275

>T228525 WP_003898789.1 NZ_CP089611:c1394040-1393747 [Mycobacterium tuberculosis]
VSDDHPYHVAITATAARDLQRLPEKIAAACVEFVFGPLLNNPHRLGKPLRNDLEGLHSARRGDYRVVYAIDDGHHRVEII
HIARRSASYRMNPCRPR

Nucleotide

• Length: 294 bp

>T228525 NZ_CP124222:6217386-6217736 [Paenibacillus dendritiformis]
TTGATTGTAAAGCGCGGCGACGTTTTTTTTGCGGATTTATCGCCCGTCGTAGGTTCAGAGCAGGGAGGGGTGCGTCCCGT
TCTCGTCATTCAGAATGATATCGGGAACCGGTTCAGCCCAACTGTCATCGTGGCGGCAATTACGGCTCAGATTCAGAAGG
CCAAATTGCCGACCCATGTTGAAATTGACGCGAAGACGCATGGCTTCGACCGCGACTCCGTCATTTTGCTGGAACAGATC
CGCACCATCGACAAACAACGGCTCACCGACAAAATAACACACTTAGATGAAGAGACGATGCACAAGGTCGATGACGGGCT
GCTTATCAGCCTCGGATTGATTGATTTTTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9766.97 Da
• Isoelectric Point: 4.8615

>AT228525 WP_003406322.1 NZ_CP089611:c1394306-1394037 [Mycobacterium tuberculosis]
MAVVPLGEVRNRLSEYVAEVELTHERITITRHGHPAAVLISADDLASIEETLEVLRTPGASEAIREGLADVAAGRFVSND
EIRNRYTAR

Nucleotide

• Length: 270 bp

>AT228525 NZ_CP124222:6217101-6217382 [Paenibacillus dendritiformis]
GTGGCTAATATTCAGAACACCAAACGAATTATGATCAGCTTGCCCGACCAACTATTGCAAGAAGTGGACGGTATCGTTGC
TATGGAAAAATCGAATCGAAGCGAGTTCATTCGCCAAGCGATGAAGTTGTATTTGCAAGAGCGCAAGAAGCGTCACATCC
GTGAATCGATGCAGCGCGGCTATATGGAAATGGCCAAAATTAACTTGACGATGGCATCCGAGGCTTTTTACGCAGAAGTC
GAAGCGGATGGCACGCTCGACCGCCTGGTTAGCGGGGTGTAG

Structures

Toxin

• AlphaFold DB: G0THS1

Antitoxin

• AlphaFold DB: G0THS2