Detailed information of TA system TA228524

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1389341..1390029
• Replicon: chromosome
• Accession: NZ_CP089611
• Organism: Mycobacterium tuberculosis strain I0004240-3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0THR7
• Locus tag: LVJ90_RS06650
• Protein ID: WP_003406304.1
• Coordinates: 1389598..1390029 (+)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0THR6
• Locus tag: LVJ90_RS06645
• Protein ID: WP_003406302.1
• Coordinates: 1389341..1389601 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ90_RS06625 (LVJ90_06615)	1384918..1385742	+	825	WP_003900298.1	trehalose ABC transporter permease SugB	-
LVJ90_RS06630 (LVJ90_06620)	1385747..1386928	+	1182	WP_003406299.1	trehalose ABC transporter ATP-binding protein SugC	-
LVJ90_RS06635 (LVJ90_06625)	1387005..1388105	-	1101	WP_003406300.1	magnesium/cobalt transporter CorA	-
LVJ90_RS06640 (LVJ90_06630)	1388276..1389265	+	990	WP_003406301.1	malate dehydrogenase	-
LVJ90_RS06645 (LVJ90_06635)	1389341..1389601	+	261	WP_003406302.1	type II toxin-antitoxin system antitoxin VapB33	Antitoxin
LVJ90_RS06650 (LVJ90_06640)	1389598..1390029	+	432	WP_003406304.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ90_RS06655 (LVJ90_06645)	1390052..1391739	-	1688	Protein_1313	PE family protein	-
LVJ90_RS06660 (LVJ90_06650)	1391919..1392779	+	861	WP_003900301.1	glycine betaine ABC transporter substrate-binding protein	-
LVJ90_RS06665 (LVJ90_06655)	1392860..1393690	-	831	WP_003898788.1	SDR family NAD(P)-dependent oxidoreductase	-
LVJ90_RS06670 (LVJ90_06660)	1393747..1394040	-	294	WP_003898789.1	type II toxin-antitoxin system mRNA interferase RelE	-
LVJ90_RS06675 (LVJ90_06665)	1394037..1394306	-	270	WP_003406322.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (2-142)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15873.08 Da
• Isoelectric Point: 5.8838

>T228524 WP_003406304.1 NZ_CP089611:1389598-1390029 [Mycobacterium tuberculosis]
VIIPDINLLLYAVITGFPQHRRAHAWWQDTVNGHTRIGLTYPALFGFLRIATSARVLAAPLPTADAIAYVREWLSQPNVD
LLTAGPRHLDIALGLLDKLGTASHLTTDVQLAAYGIEYDAEIHSSDTDFARFADLKWTDPLRE

Nucleotide

• Length: 432 bp

>T228524 NZ_CP124220:c4990051-4989683 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9918.25 Da
• Isoelectric Point: 6.4755

>AT228524 WP_003406302.1 NZ_CP089611:1389341-1389601 [Mycobacterium tuberculosis]
MRTTLTLDDDVVRLVEDAVHRERRPMKQVINDALRRALAPPVKRQEQYRLEPHESAVRSGLDLAGFNKLADELEDEALLD
ATRRAR

Nucleotide

• Length: 261 bp

>AT228524 NZ_CP124220:c4990269-4990048 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: G0THR7

Antitoxin

• AlphaFold DB: G0THR6