Detailed information of TA system TA228523

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1244475..1245043
• Replicon: chromosome
• Accession: NZ_CP089611
• Organism: Mycobacterium tuberculosis strain I0004240-3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: LVJ90_RS05975
• Protein ID: WP_003405865.1
• Coordinates: 1244669..1245043 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: LVJ90_RS05970
• Protein ID: WP_003405863.1
• Coordinates: 1244475..1244672 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ90_RS05950 (LVJ90_05945)	1240516..1241154	-	639	WP_003898730.1	lipid droplet-associated protein	-
LVJ90_RS05955 (LVJ90_05950)	1241244..1242251	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
LVJ90_RS05960 (LVJ90_05955)	1242268..1243251	-	984	WP_003898731.1	hypothetical protein	-
LVJ90_RS05965 (LVJ90_05960)	1243314..1244387	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
LVJ90_RS05970 (LVJ90_05965)	1244475..1244672	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LVJ90_RS05975 (LVJ90_05970)	1244669..1245043	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ90_RS05980 (LVJ90_05975)	1245246..1245944	+	699	WP_003898733.1	hypothetical protein	-
LVJ90_RS05985 (LVJ90_05980)	1246062..1246247	+	186	WP_003901093.1	hypothetical protein	-
LVJ90_RS05990 (LVJ90_05985)	1246174..1246449	-	276	WP_003405867.1	hypothetical protein	-
LVJ90_RS05995 (LVJ90_05990)	1246692..1247015	+	324	WP_003405871.1	putative quinol monooxygenase	-
LVJ90_RS06000 (LVJ90_05995)	1247030..1247728	-	699	WP_031646953.1	hypothetical protein	-
LVJ90_RS06005 (LVJ90_06000)	1247762..1247971	+	210	WP_003911400.1	hypothetical protein	-
LVJ90_RS06010 (LVJ90_06005)	1247923..1248693	-	771	Protein_1184	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T228523 WP_003405865.1 NZ_CP089611:1244669-1245043 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T228523 NZ_CP124220:c4964082-4963735 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGACAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT228523 WP_003405863.1 NZ_CP089611:1244475-1244672 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT228523 NZ_CP124220:c4963735-4963436 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07