Detailed information of TA system TA228509

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 701459..702105
• Replicon: chromosome
• Accession: NZ_CP089611
• Organism: Mycobacterium tuberculosis strain I0004240-3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ88
• Locus tag: LVJ90_RS03165
• Protein ID: WP_003403137.1
• Coordinates: 701459..701872 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ89
• Locus tag: LVJ90_RS03170
• Protein ID: WP_003403139.1
• Coordinates: 701869..702105 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ90_RS03145 (LVJ90_03140)	697542..699092	+	1551	WP_003403119.1	MlaD family protein	-
LVJ90_RS03150 (LVJ90_03145)	699144..699536	-	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	-
LVJ90_RS03155 (LVJ90_03150)	699533..699790	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	-
LVJ90_RS03160 (LVJ90_03155)	699973..701208	-	1236	WP_003403128.1	ATP-binding protein	-
LVJ90_RS03165 (LVJ90_03160)	701459..701872	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ90_RS03170 (LVJ90_03165)	701869..702105	-	237	WP_003403139.1	type II toxin-antitoxin system antitoxin VapB27	Antitoxin
LVJ90_RS03175 (LVJ90_03170)	702209..702715	-	507	WP_003900973.1	two component system sensor kinase HK1	-
LVJ90_RS03180 (LVJ90_03175)	702829..703359	-	531	WP_003917320.1	two component system sensor kinase HK2	-
LVJ90_RS03185 (LVJ90_03180)	703343..704038	-	696	WP_003918099.1	two-component system response regulator TcrA	-
LVJ90_RS03190 (LVJ90_03185)	704161..704472	+	312	WP_003403164.1	hypothetical protein	-
LVJ90_RS03195 (LVJ90_03190)	704544..705494	+	951	WP_003900184.1	DUF1259 domain-containing protein	-
LVJ90_RS03200 (LVJ90_03195)	705735..706319	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
LVJ90_RS03205 (LVJ90_03200)	706321..707031	+	711	Protein_635	IS607 family element RNA-guided endonuclease TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-126)

Antitoxin

• SpoVT-AbrB domain (3-43)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14786.14 Da
• Isoelectric Point: 8.1405

>T228509 WP_003403137.1 NZ_CP089611:c701872-701459 [Mycobacterium tuberculosis]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Nucleotide

• Length: 414 bp

>T228509 NZ_CP124122:c75391-74975 [Enterobacter hormaechei]
GTGAACAAGATCTATATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGTGGCCACCGTATCGTGGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCGCCGCGCCACGTCCAGCTGGTCGATGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGAT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCACTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCATGCCATCGCCGCCGGCGCGGTACTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8212.41 Da
• Isoelectric Point: 5.0351

>AT228509 WP_003403139.1 NZ_CP089611:c702105-701869 [Mycobacterium tuberculosis]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Nucleotide

• Length: 237 bp

>AT228509 NZ_CP124122:c75618-75388 [Enterobacter hormaechei]
ATGAGAACCGTATCTATTTTTAAAAACGGCAACAACCGCGCCATCCGCCTTCCCCGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGTCCCGTCCGGCCGACCTGGGGGTCGTTCGCGCAGCTCG
AAAAAGCCGATCCGGACTTTATGGCGGAGCGCGGGGACGTTGTCAGCGACGAAGGAAGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: G0TQ88

Antitoxin

• AlphaFold DB: G0TQ89