Detailed information of TA system TA228506

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-CcdA
• Location: 644527..645203
• Replicon: chromosome
• Accession: NZ_CP089611
• Organism: Mycobacterium tuberculosis strain I0004240-3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TPQ9
• Locus tag: LVJ90_RS02915
• Protein ID: WP_003898500.1
• Coordinates: 644527..644940 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TPR0
• Locus tag: LVJ90_RS02920
• Protein ID: WP_003402915.1
• Coordinates: 644937..645203 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ90_RS02885 (LVJ90_02880)	639872..640174	-	303	WP_003402896.1	DUF3349 domain-containing protein	-
LVJ90_RS02890 (LVJ90_02885)	640234..640512	-	279	WP_003402901.1	hypothetical protein	-
LVJ90_RS02895 (LVJ90_02890)	640509..641762	-	1254	WP_003402904.1	inorganic phosphate transporter	-
LVJ90_RS02900 (LVJ90_02895)	641882..642268	-	387	WP_070894909.1	VOC family protein	-
LVJ90_RS02905 (LVJ90_02900)	642331..643215	-	885	WP_003402909.1	SDR family oxidoreductase	-
LVJ90_RS02910 (LVJ90_02905)	643311..644213	-	903	WP_003915439.1	1,4-dihydroxy-2-naphthoyl-CoA synthase	-
LVJ90_RS02915 (LVJ90_02910)	644527..644940	-	414	WP_003898500.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ90_RS02920 (LVJ90_02915)	644937..645203	-	267	WP_003402915.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
LVJ90_RS02925 (LVJ90_02920)	645395..647110	-	1716	WP_003402917.1	fatty-acid--CoA ligase FadD8	-
LVJ90_RS02930 (LVJ90_02925)	647188..648792	+	1605	WP_003402920.1	amidohydrolase	-
LVJ90_RS02935 (LVJ90_02930)	648789..649769	+	981	WP_003402923.1	o-succinylbenzoate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (13-129)

Antitoxin

• Post-segregation antitoxin CcdA (19-61)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14667.78 Da
• Isoelectric Point: 7.3603

>T228506 WP_003898500.1 NZ_CP089611:c644940-644527 [Mycobacterium tuberculosis]
VRASPTSPPEQVVVDASAMVDLLARTSDRCSAVRARLARTAMHAPAHFDAEVLSALGRMQRAGALTVAYVDAALEELRQV
PVTRHGLSSLLAGAWSRRDTLRLTDALYVELAETAGLVLLTTDERLARAWPSAHAIG

Nucleotide

• Length: 414 bp

>T228506 NZ_CP124121:c3790081-3789671 [Enterobacter hormaechei]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCCTGGCGCTCACAGTGGATGTCCTTACTGCTCCACGGTCTGGTGGCGGC
ACTGGTGTTATTGGTGCCGTGGCCGCTTAGCTATACCCCTCTGTGGTTGCTATTGCTGTCGTTTGTGGTGTTTGACAGCG
TGCGCAGCCAGCGTCGAATCAACGCCCGTCAGGGTGAAATTAAGCTGCTGATGGATTCTCGCCTGCGCTGGCAGGGAAAA
GAGTGGGAGATGATGGGCGCGCCGTGGATGCTTAACACCGGGATGATGCTACGGCTGCGTCGGGTCGAGGATAACCGTCG
CCAGCACCTGTGGCTGGCCGCAGACAGTATGGATCCCGCGGAGTGGCGAGATCTGCGCCGGTTGATCGTGCAACAGCCGA
CGCAGGAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9543.79 Da
• Isoelectric Point: 4.5994

>AT228506 WP_003402915.1 NZ_CP089611:c645203-644937 [Mycobacterium tuberculosis]
MLSRRTKTIVVCTLVCMARLNVYVPDELAERARARGLNVSALTQAAISAELENSATDAWLEGLEPRSTGARHDDVLGAID
AARDEFEA

Nucleotide

• Length: 267 bp

>AT228506 NZ_CP124121:c3790328-3790062 [Enterobacter hormaechei]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCGTGCCGTCGCGGCATGCGTGAACTTGATATCTCCATCATGCCTTT
CTTCGAGTATGAGTACGACAGCTTAAGCGATGACGATAAGCGCCTGTTTGTCCGTCTGCTTGAGTCTGACGATCCGGATT
TATTCAACTGGCTGATGAATCACGGCAAGCCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CAGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: G0TPQ9

Antitoxin

• AlphaFold DB: G0TPR0