Detailed information of TA system TA228503

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Rv0299-vapB/-
• Location: 366496..367067
• Replicon: chromosome
• Accession: NZ_CP089611
• Organism: Mycobacterium tuberculosis strain I0004240-3

Toxin (Protein)

• Gene name: Rv0299
• Uniprot ID: -
• Locus tag: LVJ90_RS01585
• Protein ID: WP_003401560.1
• Coordinates: 366496..366798 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: O07227
• Locus tag: LVJ90_RS01590
• Protein ID: WP_003401563.1
• Coordinates: 366846..367067 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ90_RS01565 (LVJ90_01565)	361974..362777	-	804	WP_003401540.1	Stf0 family sulphotransferase	-
LVJ90_RS01570 (LVJ90_01570)	362787..364184	-	1398	WP_003401544.1	sulfatase	-
LVJ90_RS01575 (LVJ90_01575)	364363..366129	+	1767	WP_031648214.1	PE family protein	-
LVJ90_RS01580 (LVJ90_01580)	366272..366499	+	228	WP_003401555.1	ribbon-helix-helix protein, CopG family	-
LVJ90_RS01585 (LVJ90_01585)	366496..366798	+	303	WP_003401560.1	toxin-antitoxin system toxin	Toxin
LVJ90_RS01590 (LVJ90_01590)	366846..367067	+	222	WP_003401563.1	type II toxin-antitoxin system antitoxin VapB2	Antitoxin
LVJ90_RS01595 (LVJ90_01595)	367064..367489	+	426	WP_003401566.1	PIN domain nuclease	-
LVJ90_RS01600 (LVJ90_01600)	367625..368257	+	633	WP_003401571.1	TetR/AcrR family transcriptional regulator	-
LVJ90_RS01605 (LVJ90_01605)	368254..369162	+	909	WP_003900117.1	protochlorophyllide reductase	-
LVJ90_RS01610 (LVJ90_01610)	369170..369760	-	591	WP_229298012.1	hypothetical protein	-
LVJ90_RS01615 (LVJ90_01615)	369753..369803	-	51	Protein_319	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Ribbon-helix-helix protein, CopG (9-37)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10442.16 Da
• Isoelectric Point: 4.9609

>T228503 WP_003401560.1 NZ_CP089611:366496-366798 [Mycobacterium tuberculosis]
MIAPGDIAPRRDSEHELYVAVLSNALHRAADTGRVITCPFIPGRVPEDLLAMVVAVEQPNGTLLPELVQWLHVAALGAPL
GNAGVAALREAASVVTALLC

Nucleotide

• Length: 303 bp

>T228503 NZ_CP124121:c2308309-2307824 [Enterobacter hormaechei]
GTGGGACGTGTAACAGCACCAGAGCCCTTATCCAGCGTCCATCAACTGGCGGAGTTCGTCAGTGGAGAGGCCGTGCTTGA
CGAATGGCTAAAACAAAGAGGTCTGAAAAACCAGGCGCTCGGCGCAGCCCGAACGTTTGTCGTTTGTAAAACGGGCACGA
AGCAGGTCGCGGGATTTTACTCTCTGGCTACCGGGAGCGTTAACCATACGCAAGCGACAGGTAATCTGCGGCGTAACATG
CCGGATCCCATACCCGTGATTATACTTGCCCGTCTGGCTGTGGATGTCTCGTTACGCGGAAACGGGTTGGGAGCCGATTT
ACTCCACGATGCGGTTTTACGCTGTTATCGGGTAGCTGAGAATATCGGGGTGCGAGCGATCATGGTTCATGCACTCACTG
AGGAAGCCAAAGCGTTTTATATCCACCATGGTTTCAAAGCATCGCAGACTCAGGAACGGACACTGTTTCTCCGGCTTCCT
CAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8088.31 Da
• Isoelectric Point: 10.7177

>AT228503 WP_003401563.1 NZ_CP089611:366846-367067 [Mycobacterium tuberculosis]
MSDVLIRDIPDDVLASLDAIAARLGLSRTEYIRRRLAQDAQTARVTVTAADLRRLRGAVAGLGDPELMRQAWR

Nucleotide

• Length: 222 bp

>AT228503 NZ_CP124121:c2308563-2308297 [Enterobacter hormaechei]
ATGAAATCAGATGTTCAACTCAATCTCAGAGCCAAAGAATCCCAGCGGGCGCTTATCGATGCCGCCGCAGAGATCCTCCA
TAAATCACGCACGGACTTCATTCTGGAGATGGCCTGTCAGGCAGCCGAGAATGTGATCCTCGATCGGCGTGTATTCAATT
TTAACGATGAACAGTACGCAGAGTTCATTGACATGCTCGATGCCCCGGTCGCGGACGATCCTGCCATAGAAAAACTGCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A829CG48