Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 71605..72238 Replicon chromosome
Accession NZ_CP089611
Organism Mycobacterium tuberculosis strain I0004240-3

Toxin (Protein)


Gene name vapC Uniprot ID P9WFC0
Locus tag LVJ90_RS00355 Protein ID WP_003400580.1
Coordinates 71837..72238 (+) Length 134 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P0CW29
Locus tag LVJ90_RS00350 Protein ID WP_003400577.1
Coordinates 71605..71844 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ90_RS00335 (LVJ90_00335) 66903..68342 + 1440 WP_003901784.1 FAD-binding oxidoreductase -
LVJ90_RS00340 (LVJ90_00340) 68398..68559 + 162 WP_003899796.1 hypothetical protein -
LVJ90_RS00345 (LVJ90_00345) 68600..71538 + 2939 Protein_65 UPF0182 family protein -
LVJ90_RS00350 (LVJ90_00350) 71605..71844 + 240 WP_003400577.1 antitoxin VapB1 Antitoxin
LVJ90_RS00355 (LVJ90_00355) 71837..72238 + 402 WP_003400580.1 type II toxin-antitoxin system ribonuclease VapC1 Toxin
LVJ90_RS00360 (LVJ90_00360) 72290..74527 - 2238 WP_003899797.1 NADP-dependent isocitrate dehydrogenase -
LVJ90_RS00365 (LVJ90_00365) 74645..75214 - 570 WP_003400591.1 TetR/AcrR family transcriptional regulator -
LVJ90_RS00370 (LVJ90_00370) 75317..76228 + 912 WP_003899798.1 SDR family NAD(P)-dependent oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Pae0151-like, PIN domain

(36-124)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 134 a.a.        Molecular weight: 14339.44 Da        Isoelectric Point: 4.8887

>T228501 WP_003400580.1 NZ_CP089611:71837-72238 [Mycobacterium tuberculosis]
VDECVVDAAAVVDALAGKGASAIVLRGLLKESISNAPHLLDAEVGHALRRAVLSDEISEEQARAALDALPYLIDNRYPHS
PRLIEYTWQLRHNVTFYDALYVALATALDVPLLTGDSRLAAAPGLPCEIKLVR

Download         Length: 402 bp

>T228501 NZ_CP124121:c303531-303229 [Enterobacter hormaechei]
ATGAAGGAGATCGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAATTTAAAGGACAGGCGGGCAAAGACGATTATCGC
TTCCCGCCTCTTTAGGCTGGCAAATGGCTTAGCGGGCGACATTAGACCCGTGGGGGAAGGTATCAGTGAACTGAGGATCC
ACTTTGGCCCGGGCTACAGGATCTATTTTAAGGACCAGGGCAATAGCATCATCGTGCTGTTATGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTTATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 8717.84 Da        Isoelectric Point: 8.5960

>AT228501 WP_003400577.1 NZ_CP089611:71605-71844 [Mycobacterium tuberculosis]
MATIQVRDLPEDVAETYRRRATAAGQSLQTYMRTKLIEGVRGRDKAEAIEILEQALASTASPGISRETIEASRRELRGG

Download         Length: 240 bp

>AT228501 NZ_CP124121:c303222-302935 [Enterobacter hormaechei]
ATGCATAAATTAACACCCTACGATCCAGCCAACGCACTGCTGGATGACGAGGAAATCGCTGTGTTTATGGCTGATGCATT
AGAGACGGGTGACTCAGCGTACATTGCTAAAGCGCTGGGCGTCATCGCCCGAGCGAAAGGGATGTCGACCATTTCCCAGC
AAACGGGCCTGTCACGAGAACAACTGTATCGATCATTCAGTGATAAGGGGAACCCAACGCTCAAAACCACGCTGGCGGTC
ATGAAAGCATTGGGCCTTGGGTTAACAATCAAACCCTCTGGGGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BR82

Antitoxin

Source ID Structure
AlphaFold DB A0A829CHM7

References