Detailed information of TA system TA228499

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-PHD
• Location: 3808248..3808915
• Replicon: chromosome
• Accession: NZ_CP089610
• Organism: Mycobacterium tuberculosis strain I0004290-8

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: O50411
• Locus tag: LVJ91_RS17920
• Protein ID: WP_003417916.1
• Coordinates: 3808248..3808640 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0E8U8S7
• Locus tag: LVJ91_RS17925
• Protein ID: WP_003912214.1
• Coordinates: 3808640..3808915 (-)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ91_RS17900	3803620..3805230	-	1611	WP_003417910.1	1-deoxy-D-xylulose-5-phosphate synthase N-terminal domain-containing protein	-
LVJ91_RS17905	3805175..3805456	-	282	WP_003914149.1	1-deoxy-D-xylulose-5-phosphate synthase N-terminal domain-containing protein	-
LVJ91_RS17910 (LVJ91_17920)	3805453..3806442	-	990	WP_003417912.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
LVJ91_RS17915 (LVJ91_17925)	3806442..3807494	-	1053	WP_003900678.1	polyprenyl synthetase family protein	-
LVJ91_RS17920 (LVJ91_17930)	3808248..3808640	-	393	WP_003417916.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ91_RS17925 (LVJ91_17935)	3808640..3808915	-	276	WP_003912214.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LVJ91_RS17930 (LVJ91_17940)	3809097..3810468	+	1372	Protein_3542	ISNCY family transposase	-
LVJ91_RS17935 (LVJ91_17945)	3810658..3812853	+	2196	WP_010886168.1	PE family protein	-
LVJ91_RS17940 (LVJ91_17950)	3812924..3813796	-	873	WP_003417929.1	3-hydroxyacyl-thioester dehydratase HtdY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3809791..3810468	677

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-120)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-53)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14316.74 Da
• Isoelectric Point: 10.7249

>T228499 WP_003417916.1 NZ_CP089610:c3808640-3808248 [Mycobacterium tuberculosis]
MAAIYLDSSAIVKLAVREPESDALRRYLRTRHPRVSSALARAEVMRALLDKGESARKAGRRALAHLDLLRVDKRVLDLAG
GLLPFELRTLDAIHLATAQRLGVDLGRLCTYDDRMRDAAKTLGMAVIAPS

Nucleotide

• Length: 393 bp

>T228499 NZ_CP123994:c1453484-1452738 [Bacillus inaquosorum]
ATGCTTTTATTCTTTCAGATCATGGTCTGGTGCGTCATGGCCGGATTAGGCTTATACGTGTACGCCACGTGGCGGTTCGA
AGCGAAGGTCAAAGAAAAAATGTCCGCCATCCGGAAAACATGGTACTTGCTGTTTGTTCTGGGCGCTATGGTATACTGGA
CATATGAGCCCTCTTCCCTATTTACCCACTGGGAACGGTACCTCATTGTCGCTGTCAGTTTCGCTTTAATTGACGCTTTC
ATCTTCTTAAGCGCATACGTCAAAAAACTGGCTGGCAGCGAACTGGAAACAGACACAAGAGAAATCCTTGAAGAAAACAA
CGAAATGCTCCACATGTATCTCAATCGGCTGAAAACATACCAATACCTATTGAAAAACGAACCGATCCATGTTTATTATG
GAAGTATAGATGCATATGCTGAAGGTATTGATAAGCTGCTGAAAACCTATGCCGATAAAATGAATTTAACAGCCTCCCTT
TGTCACTATTCGACACAGGCTGATAAAGACCGGTTAACCGAGCATATGGACGATCCGGCAGACGTGCAAAACCGGCTTGA
TCGAAAGGATGTTTATTACGACCAATACGGAAAAATGGTTCTCATTCCTTTTACCATCGAGACACAGAGCTATGTCATCA
AGCTGACGTCTGACAGCATTGTCACTGAATTTGATTATTTGCTTTTCACATCATTAACGAGCATATATGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGAAGGATAA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 9966.35 Da
• Isoelectric Point: 4.5332

>AT228499 WP_003912214.1 NZ_CP089610:c3808915-3808640 [Mycobacterium tuberculosis]
MTSVGVRALRQRASELLRRVEAGETIEITDRGRPVALLSPLPQGGPYEQLLASGEIERATLDVVDLPEPLDLDAGVELPS
VTLARLREHER

Nucleotide

• Length: 276 bp

>AT228499 NZ_CP123994:c1452738-1452568 [Bacillus inaquosorum]
ATGGAACGCGCGTTTCAAAACGGATGCGAGCCCCGAGCGGCGAAGCCGTTTAAAATCCTGAAAAAACGTTCAACCACCAG
TGTTGCAAGCTATCAAGTCAGTCCGCATACAGCAAGAATCTTTAAAGAAAACGAACGGCTGATTGACGAGTATAAACGAA
AAAAAGCATGA

Structures

Toxin

• AlphaFold DB: A0A7U4BXS8

Antitoxin

• AlphaFold DB: A0A0E8U8S7