Detailed information of TA system TA228494

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-Phd
• Location: 3186822..3187370
• Replicon: chromosome
• Accession: NZ_CP089610
• Organism: Mycobacterium tuberculosis strain I0004290-8

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G0TFG5
• Locus tag: LVJ91_RS15185
• Protein ID: WP_003414602.1
• Coordinates: 3187107..3187370 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: O33347
• Locus tag: LVJ91_RS15180
• Protein ID: WP_003414599.1
• Coordinates: 3186822..3187103 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ91_RS15155 (LVJ91_15160)	3182445..3183302	-	858	WP_003899513.1	type I methionyl aminopeptidase	-
LVJ91_RS15160 (LVJ91_15165)	3183344..3183928	-	585	WP_003899514.1	DUF1707 domain-containing protein	-
LVJ91_RS15165 (LVJ91_15170)	3184032..3184280	+	249	WP_003913411.1	antitoxin VapB23	-
LVJ91_RS15170 (LVJ91_15175)	3184277..3184657	+	381	WP_003414592.1	type II toxin-antitoxin system VapC family toxin	-
LVJ91_RS15175 (LVJ91_15180)	3184739..3186550	-	1812	WP_003906921.1	penicillin-binding protein	-
LVJ91_RS15180 (LVJ91_15185)	3186822..3187103	+	282	WP_003414599.1	type II toxin-antitoxin system antitoxin RelF	Antitoxin
LVJ91_RS15185 (LVJ91_15190)	3187107..3187370	+	264	WP_003414602.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LVJ91_RS15190 (LVJ91_15195)	3187743..3188597	-	855	WP_070894445.1	GNAT family N-acetyltransferase	-
LVJ91_RS15195 (LVJ91_15200)	3188653..3189816	-	1164	WP_003899517.1	flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase	-
LVJ91_RS15200 (LVJ91_15205)	3189833..3191047	-	1215	WP_003899518.1	zinc metalloprotease Rip	-
LVJ91_RS15205 (LVJ91_15210)	3191055..3192296	-	1242	WP_003414613.1	1-deoxy-D-xylulose-5-phosphate reductoisomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10204.82 Da
• Isoelectric Point: 11.5078

>T228494 WP_003414602.1 NZ_CP089610:3187107-3187370 [Mycobacterium tuberculosis]
VPYTVRFTTTARRDLHKLPPRILAAVVEFAFGDLSREPLRVGKPLRRELAGTFSARRGTYRLLYRIDDEHTTVVILRVDH
RADIYRR

Nucleotide

• Length: 264 bp

>T228494 NZ_CP123992:12251-12532 [Acinetobacter baumannii]
ATGGCTATCAAACTTCGTAAATGGGATAGTGCTGAGCATCTTAAAACAGAAGAAGATATGCAAGCTTACCTACAAGCCTG
TATCGATGAATCTAATGGTGATGCTGCATTCATTGCTAAAGCTTTAGGTAACATTGCTAAAGCTAAAGGAATGGCTCAAT
TATCACGAGATACAGGTCTAGGCCGAGAAAGTCTTTACAAAGCACTTTCTGGTGATGTTAACCCTAGTTTTGATACTGTG
ATTAAAGTTGTAAAAGCACTGAATTTACGCTTAGCAATTTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10183.40 Da
• Isoelectric Point: 4.3440

>AT228494 WP_003414599.1 NZ_CP089610:3186822-3187103 [Mycobacterium tuberculosis]
MRILPISTIKGKLNEFVDAVSSTQDQITITKNGAPAAVLVGADEWESLQETLYWLAQPGIRESIAEADADIASGRTYGED
EIRAEFGVPRRPH

Nucleotide

• Length: 282 bp

>AT228494 NZ_CP123992:11948-12250 [Acinetobacter baumannii]
ATGTACTCAATATATACCACAGAAGCCTTTGATGATTGGTTTACCAAGTTAAAAGATCAACAGGCAAAAAGACGCATACA
AGTCCGAATTGATCGGGTTGAAGATGGGAACTTTGGAGATACTGAACCAGTCGGTGAAGGTGTTTCTGAGTTACGTTTCT
TCTTTGGACCTGGCTATAGGATTTATTACTGCAAGCAAGGGCAAAGGGTCGTTATTCTCTTAGCAGGCGGAGATAAGTCT
ACGCAAAGTAAAGATATAAAACTTGCCCTACAATTAGCACAAGATTTAGAGGAGGAGCTGTAA

Structures

Toxin

• PDB: 3G5O

Antitoxin

• AlphaFold DB: A0A7U4BWP8