Detailed information of TA system TA228493

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN_Sll0205-like-Phd
• Location: 3146015..3146619
• Replicon: chromosome
• Accession: NZ_CP089610
• Organism: Mycobacterium tuberculosis strain I0004290-8

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P71623
• Locus tag: LVJ91_RS14985
• Protein ID: WP_003414492.1
• Coordinates: 3146015..3146407 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A829CBY8
• Locus tag: LVJ91_RS14990
• Protein ID: WP_003414495.1
• Coordinates: 3146404..3146619 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ91_RS14955 (LVJ91_14965)	3141165..3141953	-	789	WP_003917092.1	CRISPR-associated endoribonuclease Cas6	-
LVJ91_RS14960 (LVJ91_14970)	3142287..3142832	-	546	WP_003913758.1	DUF1802 family protein	-
LVJ91_RS14965 (LVJ91_14975)	3143104..3143988	-	885	WP_003414409.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
LVJ91_RS14970 (LVJ91_14980)	3143991..3144878	-	888	WP_003414414.1	type IV toxin-antitoxin system AbiEi family antitoxin	-
LVJ91_RS14975 (LVJ91_14985)	3145183..3145728	-	546	WP_003899500.1	DUF1802 family protein	-
LVJ91_RS14980 (LVJ91_14990)	3145725..3145994	-	270	WP_003414489.1	DUF2277 family protein	-
LVJ91_RS14985 (LVJ91_14995)	3146015..3146407	-	393	WP_003414492.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ91_RS14990 (LVJ91_15000)	3146404..3146619	-	216	WP_003414495.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LVJ91_RS14995 (LVJ91_15005)	3146666..3147415	+	750	WP_003414497.1	enoyl-CoA hydratase	-
LVJ91_RS15000 (LVJ91_15010)	3147494..3148574	-	1081	Protein_2961	ABC transporter ATP-binding protein	-
LVJ91_RS15005 (LVJ91_15015)	3148567..3149877	-	1311	WP_003414501.1	ABC transporter substrate-binding protein	-
LVJ91_RS15010 (LVJ91_15020)	3149880..3150707	-	828	WP_003414504.1	carbohydrate ABC transporter permease	-
LVJ91_RS15015 (LVJ91_15025)	3150704..3151615	-	912	WP_003414505.1	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• VapC-like Sll0205 protein, PIN domain (5-130)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-46)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14610.74 Da
• Isoelectric Point: 6.7594

>T228493 WP_003414492.1 NZ_CP089610:c3146407-3146015 [Mycobacterium tuberculosis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Nucleotide

• Length: 393 bp

>T228493 NZ_CP123992:11948-12250 [Acinetobacter baumannii]
ATGTACTCAATATATACCACAGAAGCCTTTGATGATTGGTTTACCAAGTTAAAAGATCAACAGGCAAAAAGACGCATACA
AGTCCGAATTGATCGGGTTGAAGATGGGAACTTTGGAGATACTGAACCAGTCGGTGAAGGTGTTTCTGAGTTACGTTTCT
TCTTTGGACCTGGCTATAGGATTTATTACTGCAAGCAAGGGCAAAGGGTCGTTATTCTCTTAGCAGGCGGAGATAAGTCT
ACGCAAAGTAAAGATATAAAACTTGCCCTACAATTAGCACAAGATTTAGAGGAGGAGCTGTAA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7511.55 Da
• Isoelectric Point: 4.9732

>AT228493 WP_003414495.1 NZ_CP089610:c3146619-3146404 [Mycobacterium tuberculosis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Nucleotide

• Length: 216 bp

>AT228493 NZ_CP123992:12251-12532 [Acinetobacter baumannii]
ATGGCTATCAAACTTCGTAAATGGGATAGTGCTGAGCATCTTAAAACAGAAGAAGATATGCAAGCTTACCTACAAGCCTG
TATCGATGAATCTAATGGTGATGCTGCATTCATTGCTAAAGCTTTAGGTAACATTGCTAAAGCTAAAGGAATGGCTCAAT
TATCACGAGATACAGGTCTAGGCCGAGAAAGTCTTTACAAAGCACTTTCTGGTGATGTTAACCCTAGTTTTGATACTGTG
ATTAAAGTTGTAAAAGCACTGAATTTACGCTTAGCAATTTAA

Structures

Toxin

• AlphaFold DB: A0A7U4BWM8

Antitoxin

• AlphaFold DB: A0A829CBY8