Detailed information of TA system TA228491

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3119951..3120521
• Replicon: chromosome
• Accession: NZ_CP089610
• Organism: Mycobacterium tuberculosis strain I0004290-8

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: P71650
• Locus tag: LVJ91_RS14840
• Protein ID: WP_003414166.1
• Coordinates: 3119951..3120307 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: P0CL61
• Locus tag: LVJ91_RS14845
• Protein ID: WP_003901465.1
• Coordinates: 3120291..3120521 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ91_RS14820 (LVJ91_14830)	3115403..3117091	-	1689	WP_003414155.1	alpha/beta hydrolase family protein	-
LVJ91_RS14825 (LVJ91_14835)	3117095..3117421	-	327	WP_003414157.1	hypothetical protein	-
LVJ91_RS14830 (LVJ91_14840)	3117594..3118181	+	588	WP_023641719.1	DUF3558 family protein	-
LVJ91_RS14835 (LVJ91_14845)	3118200..3119849	+	1650	WP_003899485.1	CocE/NonD family hydrolase	-
LVJ91_RS14840 (LVJ91_14850)	3119951..3120307	-	357	WP_003414166.1	type II toxin-antitoxin system toxin endoribonuclease MazF9	Toxin
LVJ91_RS14845 (LVJ91_14855)	3120291..3120521	-	231	WP_003901465.1	type II toxin-antitoxin system antitoxin MazE9	Antitoxin
LVJ91_RS14850 (LVJ91_14860)	3120564..3121607	-	1044	WP_003414172.1	DUF2293 domain-containing protein	-
LVJ91_RS14855 (LVJ91_14865)	3121606..3122073	+	468	WP_003414177.1	DUF1778 domain-containing protein	-
LVJ91_RS14860 (LVJ91_14870)	3122249..3122503	-	255	WP_003917684.1	hypothetical protein	-
LVJ91_RS14865 (LVJ91_14875)	3122651..3123055	+	405	WP_009938577.1	hypothetical protein	-
LVJ91_RS14870 (LVJ91_14880)	3123052..3123243	+	192	WP_003414184.1	hypothetical protein	-
LVJ91_RS14875 (LVJ91_14885)	3123460..3123720	+	261	Protein_2936	transposase	-
LVJ91_RS14880 (LVJ91_14890)	3124830..3125087	+	258	WP_003899489.1	hypothetical protein	-
LVJ91_RS14885 (LVJ91_14895)	3125192..3125503	+	312	WP_003414190.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-29)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12858.73 Da
• Isoelectric Point: 9.1962

>T228491 WP_003414166.1 NZ_CP089610:c3120307-3119951 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Nucleotide

• Length: 357 bp

>T228491 NZ_CP123983:1977492-1977680 [Staphylococcus haemolyticus]
ATGAGTTTGCATTTAACTATATTGTTATGGCTTGGTATCATTTTTGTAATAGCAGCCTCAATTATATTGGGCTTATTACT
TAAATCTAAAAAAGAAGAACGTAAAGAATCCTATTTAGGTTTTACCGTAATATTTTATATTTTTGGATTTGCTTTACTCA
TCTACGTATTCATATTTGGTATATTATAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8231.01 Da
• Isoelectric Point: 4.1809

>AT228491 WP_003901465.1 NZ_CP089610:c3120521-3120291 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Nucleotide

• Length: 231 bp

>AT228491 NZ_CP123983:1977212-1977469 [Staphylococcus haemolyticus]
ATGAATAATAATAAAATTTTTTGGATAAATATTTTTATTACGCTATTATTTTTGGGTTTCAATATCATTGTTACTTACAA
TGCAGAATTAGATGATTTCTTCTGGCTAATTCCAGGACTTACAATTAGTGGTATCACAATTGTACTAAGCTTAAGTACTG
CGCTCATTTGCAAGAACCTGGTTAGCGAAGTTATTTTTCTAATCAATATTGCTATGCTTTTATACTACATTTATCCTATA
GTATATACATTCTTTTAA

Structures

Toxin

• PDB: 6L2A
• PDB: 5HJZ
• PDB: 6KYT
• PDB: 6L29
• PDB: 7DU5
• PDB: 6KYS

Antitoxin

• PDB: 7DU5
• PDB: 6KYT
• AlphaFold DB: A0A7U4FBW3