Detailed information of TA system TA228487

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2941157..2941871
• Replicon: chromosome
• Accession: NZ_CP089610
• Organism: Mycobacterium tuberculosis strain I0004290-8

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQK0
• Locus tag: LVJ91_RS13790
• Protein ID: WP_003413460.1
• Coordinates: 2941431..2941871 (+)
• Length: 147 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ20
• Locus tag: LVJ91_RS13785
• Protein ID: WP_003413456.1
• Coordinates: 2941157..2941444 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ91_RS13750 (LVJ91_13755)	2936579..2936824	+	246	WP_003413429.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
LVJ91_RS13755 (LVJ91_13760)	2936821..2937225	+	405	WP_003899389.1	type II toxin-antitoxin system VapC family toxin	-
LVJ91_RS13760 (LVJ91_13765)	2937442..2938062	+	621	WP_003413441.1	DUF4178 domain-containing protein	-
LVJ91_RS13765 (LVJ91_13770)	2938073..2938567	+	495	WP_003413444.1	DUF2617 family protein	-
LVJ91_RS13770 (LVJ91_13775)	2938564..2938995	+	432	WP_003899390.1	DUF4247 domain-containing protein	-
LVJ91_RS13775 (LVJ91_13780)	2939020..2939478	+	459	WP_003413451.1	DUF350 domain-containing protein	-
LVJ91_RS13780 (LVJ91_13785)	2939475..2941046	+	1572	WP_003899392.1	polyamine aminopropyltransferase	-
LVJ91_RS13785 (LVJ91_13790)	2941157..2941444	+	288	WP_003413456.1	antitoxin VapB41	Antitoxin
LVJ91_RS13790 (LVJ91_13795)	2941431..2941871	+	441	WP_003413460.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ91_RS13795 (LVJ91_13800)	2941892..2942647	-	756	WP_003413464.1	YebC/PmpR family DNA-binding transcriptional regulator	-
LVJ91_RS13800 (LVJ91_13805)	2942780..2943376	-	597	WP_003413465.1	pyridoxal 5'-phosphate synthase glutaminase subunit PdxT	-
LVJ91_RS13805 (LVJ91_13810)	2943384..2944229	-	846	WP_003413466.1	acyl-CoA thioesterase II	-
LVJ91_RS13810 (LVJ91_13815)	2944258..2945157	-	900	WP_003413468.1	pyridoxal 5'-phosphate synthase lyase subunit PdxS	-
LVJ91_RS13815 (LVJ91_13820)	2945285..2945959	+	675	WP_003413471.1	pyridoxamine 5'-phosphate oxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-146)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 147 a.a.
• Molecular weight: 16026.38 Da
• Isoelectric Point: 6.8599

>T228487 WP_003413460.1 NZ_CP089610:2941431-2941871 [Mycobacterium tuberculosis]
MLLCDTNIWLALALSGHVHHRASRAWLDTINAPGVIHFCRATQQSLLRLLTNRTVLGAYGSPPLTNREAWAAYAAFLDDD
RIVLAGAEPDGLEAQWRAFAVRQSPAPKVWMDAYLAAFALTGGFELVTTDTAFTQYGGIELRLLAK

Nucleotide

• Length: 441 bp

>T228487 NZ_CP123979:2040713-2040901 [Staphylococcus haemolyticus]
ATGAGTTTGCATTTAACTATATTGTTATGGCTTGGTATCATTTTTGTAATAGCAGCCTCAATTATATTGGGCTTATTACT
TAAATCTAAAAAAGAAGAACGTAAAGAATCCTATTTAGGTTTTACCGTAATATTTTATATTTTTGGATTTGCTTTACTCA
TCTACGTATTCATATTTGGTATATTATAG

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10453.98 Da
• Isoelectric Point: 7.2797

>AT228487 WP_003413456.1 NZ_CP089610:2941157-2941444 [Mycobacterium tuberculosis]
MKTTLDLPDELMRAIKVRAAQQGRKMKDVVTELLRSGLSQTHSGAPIPTPRRVQLPLVHCGGAATREQEMTPERVAAALL
DQEAQWWSGHDDAAL

Nucleotide

• Length: 288 bp

>AT228487 NZ_CP123979:2040433-2040690 [Staphylococcus haemolyticus]
ATGAATAATAATAAAATTTTTTGGATAAATATTTTTATTACGCTATTATTTTTGGGTTTCAATATCATTGTTACTTACAA
TGCAGAATTAGATGATTTCTTCTGGCTAATTCCAGGACTTACAATTAGTGGTATCACAATTGTACTAAGCTTAAGTACTG
CGCTCATTTGCAAGAACCTGGTTAGCGAAGTTATTTTTCTAATCAATATTGCTATGCTTTTATACTACATTTATCCTATA
GTATATACATTCTTTTAA

Structures

Toxin

• AlphaFold DB: G0TQK0

Antitoxin

• AlphaFold DB: A0A7U4BWB8