Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2880818..2881455 Replicon chromosome
Accession NZ_CP089610
Organism Mycobacterium tuberculosis strain I0004290-8

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag LVJ91_RS13525 Protein ID WP_003413180.1
Coordinates 2880818..2881213 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag LVJ91_RS13530 Protein ID WP_003413183.1
Coordinates 2881210..2881455 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ91_RS13485 (LVJ91_13490) 2876221..2877432 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
LVJ91_RS13490 (LVJ91_13495) 2877559..2878218 + 660 WP_003900846.1 LppA family lipoprotein -
LVJ91_RS13495 (LVJ91_13500) 2878215..2878877 + 663 WP_003900848.1 LppA family lipoprotein -
LVJ91_RS13500 (LVJ91_13505) 2878874..2879152 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
LVJ91_RS13505 (LVJ91_13510) 2879245..2879658 + 414 WP_003413164.1 PIN domain nuclease -
LVJ91_RS13510 (LVJ91_13515) 2879697..2879954 + 258 WP_003413167.1 CopG family transcriptional regulator -
LVJ91_RS13515 (LVJ91_13520) 2879951..2880328 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
LVJ91_RS13520 (LVJ91_13525) 2880344..2880718 - 375 WP_003413177.1 hypothetical protein -
LVJ91_RS13525 (LVJ91_13530) 2880818..2881213 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
LVJ91_RS13530 (LVJ91_13535) 2881210..2881455 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
LVJ91_RS13535 (LVJ91_13540) 2881866..2882285 - 420 WP_003413190.1 A24 family peptidase -
LVJ91_RS13540 (LVJ91_13545) 2882297..2883106 - 810 WP_003413193.1 shikimate dehydrogenase -
LVJ91_RS13545 (LVJ91_13550) 2883103..2884356 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
LVJ91_RS13550 (LVJ91_13555) 2884349..2884861 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T228486 WP_003413180.1 NZ_CP089610:c2881213-2880818 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T228486 NZ_CP123979:1109117-1109266 [Staphylococcus haemolyticus]
ATGACTAAAAATAAAGATTCAGAATTAAATTATCATGAGGAAGAAAATGCGATGGTTCAAGATTTAGATGACTTGAAAAC
CTTGGGTAAAGAAATGGAACAAATTTCTGAAGAAAACGACGAGGACAAGTTGAATCAATCGCATGAGTAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT228486 WP_003413183.1 NZ_CP089610:c2881455-2881210 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT228486 NZ_CP123979:1108495-1109094 [Staphylococcus haemolyticus]
ATGGCAATGAACTTTAAAGTATTTAATGATGTAGAACATGTAGCTGAGTACACAGCAGATATCATTAGAAAGCAATTTAA
TAATAACCCTACAACTATTGCGGGAATTCATTTAACAAAGGATGCTGCACCTGTTTTAGATGAGTTAAAGAAAGATGTAG
ATCATAATGCGGTTGATTTTAGTCAAGTAAATATCTTAGATTATGACGATAATCGCTCTTACTATGAAGCATTAGGTGTA
CCTGCTAGTCAAATTTATCCAATTAATCTTGACGATGATGCAGAATCTTTAATTGATGACAAAATTAAAACTAAAGAAAA
TAAAGGTAAATTAATTTTACAAGTTACTTCTATTGATGAATCAGGTAGTTTAAATGTAAATGTAAGACAAGGATTATTAA
AAGCGCGTGAAGTAGTATTAGTTGTGACTGGTGCTAATAAACGTGAAGTTGTTAAAAAACTTTATGAAGAAAATGGTAAA
TCTAGCTTTGAACCATCTGATTTAAAAGCTCATAGAATGGTAACAGTTGTTTTAGATAGAGCAGCTGCAGAAGGTTTACC
TGAGGATGTTAAAGAATATTTCACTGCTAGATTTGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References