Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2879697..2880328 Replicon chromosome
Accession NZ_CP089610
Organism Mycobacterium tuberculosis strain I0004290-8

Toxin (Protein)


Gene name vapC Uniprot ID G0TQ28
Locus tag LVJ91_RS13515 Protein ID WP_003413174.1
Coordinates 2879951..2880328 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P95006
Locus tag LVJ91_RS13510 Protein ID WP_003413167.1
Coordinates 2879697..2879954 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ91_RS13480 (LVJ91_13485) 2875611..2875925 + 315 WP_009937839.1 hypothetical protein -
LVJ91_RS13485 (LVJ91_13490) 2876221..2877432 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
LVJ91_RS13490 (LVJ91_13495) 2877559..2878218 + 660 WP_003900846.1 LppA family lipoprotein -
LVJ91_RS13495 (LVJ91_13500) 2878215..2878877 + 663 WP_003900848.1 LppA family lipoprotein -
LVJ91_RS13500 (LVJ91_13505) 2878874..2879152 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
LVJ91_RS13505 (LVJ91_13510) 2879245..2879658 + 414 WP_003413164.1 PIN domain nuclease -
LVJ91_RS13510 (LVJ91_13515) 2879697..2879954 + 258 WP_003413167.1 CopG family transcriptional regulator Antitoxin
LVJ91_RS13515 (LVJ91_13520) 2879951..2880328 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin Toxin
LVJ91_RS13520 (LVJ91_13525) 2880344..2880718 - 375 WP_003413177.1 hypothetical protein -
LVJ91_RS13525 (LVJ91_13530) 2880818..2881213 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 -
LVJ91_RS13530 (LVJ91_13535) 2881210..2881455 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 -
LVJ91_RS13535 (LVJ91_13540) 2881866..2882285 - 420 WP_003413190.1 A24 family peptidase -
LVJ91_RS13540 (LVJ91_13545) 2882297..2883106 - 810 WP_003413193.1 shikimate dehydrogenase -
LVJ91_RS13545 (LVJ91_13550) 2883103..2884356 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
LVJ91_RS13550 (LVJ91_13555) 2884349..2884861 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13728.72 Da        Isoelectric Point: 4.4687

>T228485 WP_003413174.1 NZ_CP089610:2879951-2880328 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Download         Length: 378 bp

>T228485 NZ_CP123979:964565-964930 [Staphylococcus haemolyticus]
ATGATTAGAAGAGGCGATGTATATTTAGCTGATTTGTCACCAGTACAGGGCTCAGAACAAGGGGGAGTCAGACCTGTAGT
TATCATTCAAAACGATACTGGTAACAAATATAGTCCTACCGTAATCGTTGCTGCGATTACGGGAAGGATTAATAAAGCGA
AAATACCCACACATGTTGAAATCGAGAAAAAGAAATACAGACTTGATAAAGATTCAGTTATTCTACTCGAGCAAATTAGA
ACATTAGATAAAAAACGACTAAAAGAAAAATTAACATATCTATCTGATGAGAAAATGCAAGAAGTGGATGAAGCGTTAGA
CATTAGTCTAGGATTGCATGATGAAGTTAAAACGCAAAACACATAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9453.63 Da        Isoelectric Point: 10.6555

>AT228485 WP_003413167.1 NZ_CP089610:2879697-2879954 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Download         Length: 258 bp

>AT228485 NZ_CP123979:964398-964568 [Staphylococcus haemolyticus]
ATGCTTACTTTCAATCAAAATAGAAGTCACAGTCTTGAACAATCTTTAAAAGAAGGCTATGCACAAATGGCCGAATTAAA
TCTCTCCCTAGCGACAGAAGCTTTTCCTCTAGAATGCGAAGCTTGTGATTGCAACGAAACGTACCTTATTTCTAATTATA
AAAGTGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQ28

Antitoxin

Source ID Structure
AlphaFold DB A0A829C9W5

References