Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2819174..2819826 Replicon chromosome
Accession NZ_CP089610
Organism Mycobacterium tuberculosis strain I0004290-8

Toxin (Protein)


Gene name vapC Uniprot ID G0TPX1
Locus tag LVJ91_RS13225 Protein ID WP_003412752.1
Coordinates 2819401..2819826 (+) Length 142 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ24
Locus tag LVJ91_RS13220 Protein ID WP_003412749.1
Coordinates 2819174..2819395 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ91_RS13205 (LVJ91_13210) 2817414..2817620 - 207 WP_003899347.1 hypothetical protein -
LVJ91_RS13210 (LVJ91_13215) 2817756..2818379 + 624 WP_003900858.1 TIGR00725 family protein -
LVJ91_RS13215 (LVJ91_13220) 2818369..2819121 + 753 WP_003900859.1 hypothetical protein -
LVJ91_RS13220 (LVJ91_13225) 2819174..2819395 + 222 WP_003412749.1 antitoxin Antitoxin
LVJ91_RS13225 (LVJ91_13230) 2819401..2819826 + 426 WP_003412752.1 type II toxin-antitoxin system VapC family toxin Toxin
LVJ91_RS13230 (LVJ91_13235) 2819849..2821030 - 1182 WP_003899351.1 dihydrolipoamide acetyltransferase family protein -
LVJ91_RS13235 (LVJ91_13240) 2821027..2822073 - 1047 WP_003412757.1 3-methyl-2-oxobutanoate dehydrogenase subunit beta -
LVJ91_RS13240 (LVJ91_13245) 2822084..2823187 - 1104 WP_003412761.1 pyruvate dehydrogenase (acetyl-transferring) E1 component subunit alpha -
LVJ91_RS13245 (LVJ91_13250) 2823446..2824267 - 822 WP_003412766.1 citrate (pro-3S)-lyase subunit beta -
LVJ91_RS13250 (LVJ91_13255) 2824264..2824821 - 558 WP_003412768.1 MaoC family dehydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-121)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 142 a.a.        Molecular weight: 15392.89 Da        Isoelectric Point: 9.2386

>T228482 WP_003412752.1 NZ_CP089610:2819401-2819826 [Mycobacterium tuberculosis]
VALLDVNALVALAWDSHIHHARIREWFTANATLGWATCPLTEAGFVRVSTNPKVLPSAIGIADARRVLVALRAVGGHRFL
ADDVSLVDDDVPLIVGYRQVTDAHLLTLARRRGVRLVTFDAGVFTLAQQRPKTPVELLTIL

Download         Length: 426 bp

>T228482 NZ_CP123977:516933-517283 [Bacillus subtilis subsp. subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 7864.93 Da        Isoelectric Point: 4.5258

>AT228482 WP_003412749.1 NZ_CP089610:2819174-2819395 [Mycobacterium tuberculosis]
VRTTLDLDDDVIAAARELASSQRRSLGSVISELARRGLMPGRVEADDGLPVIRVPAGTPPITPEMVRRALDED

Download         Length: 222 bp

>AT228482 NZ_CP123977:516647-516928 [Bacillus subtilis subsp. subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TPX1

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW03

References