228474

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC(toxin)
Location 2230161..2230849 Replicon chromosome
Accession NZ_CP089610
Organism Mycobacterium tuberculosis strain I0004290-8

Toxin (Protein)


Gene name vapC Uniprot ID P0A653
Locus tag LVJ91_RS10415 Protein ID WP_003409958.1
Coordinates 2230161..2230580 (-) Length 140 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ28
Locus tag LVJ91_RS10420 Protein ID WP_003409968.1
Coordinates 2230589..2230849 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ91_RS10395 (LVJ91_10410) 2225656..2226504 + 849 WP_003409942.1 class I SAM-dependent methyltransferase -
LVJ91_RS10400 (LVJ91_10415) 2226467..2227912 - 1446 WP_003899115.1 APC family permease -
LVJ91_RS10405 (LVJ91_10420) 2228091..2228777 - 687 WP_003409954.1 immunoprotective protein Mpt64 -
LVJ91_RS10410 (LVJ91_10425) 2228968..2229936 - 969 WP_003899116.1 class 1b ribonucleoside-diphosphate reductase subunit beta -
LVJ91_RS10415 (LVJ91_10430) 2230161..2230580 - 420 WP_003409958.1 type II toxin-antitoxin system VapC family toxin Toxin
LVJ91_RS10420 (LVJ91_10435) 2230589..2230849 - 261 WP_003409968.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LVJ91_RS10425 (LVJ91_10440) 2230992..2232668 + 1677 WP_012054305.1 PecA family PE domain-processing aspartic protease -
LVJ91_RS10430 (LVJ91_10445) 2232656..2233309 - 654 WP_003409976.1 cutinase Cfp21 -
LVJ91_RS10435 (LVJ91_10450) 2233424..2233654 + 231 WP_003409980.1 DUF167 domain-containing protein -
LVJ91_RS10440 (LVJ91_10455) 2233739..2234650 - 912 WP_003409986.1 ArgP/LysG family DNA-binding transcriptional regulator -
LVJ91_RS10445 (LVJ91_10460) 2234759..2235358 + 600 WP_003409989.1 L-lysine exporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-125)

Antitoxin

Antitoxin VapB-like

(2-81)


Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 14724.89 Da        Isoelectric Point: 7.9535

>T228474 WP_003409958.1 NZ_CP089610:c2230580-2230161 [Mycobacterium tuberculosis]
MIVDTSAVVALVQGERPHATLVAAALAGAHSPVMSAPTVAECLIVLTARHGPVARTIFERLRSEIGLSVSSFTAEHAAAT
QRAFLRYGKGRHRAALNFGDCMTYATAQLGHQPLLAVGNDFPQTDLEFRGVVGYWPGVA

Download         Length: 420 bp

>T228474 NZ_CP123963:3650497-3650775 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCATATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9528.88 Da        Isoelectric Point: 5.1007

>AT228474 WP_003409968.1 NZ_CP089610:c2230849-2230589 [Mycobacterium tuberculosis]
VALNIKDPEVDRLAAELADRLHTSKTAAIRHALSAQLAFLESRAGDREAQLLDILRTEIWPLLADRSPITKLEREQILGY
DPATGV

Download         Length: 261 bp

>AT228474 NZ_CP123963:3650234-3650494 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829C4H9

Antitoxin

Source ID Structure
AlphaFold DB A0A829C483

References