Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2199391..2200094 Replicon chromosome
Accession NZ_CP089610
Organism Mycobacterium tuberculosis strain I0004290-8

Toxin (Protein)


Gene name mazF Uniprot ID G0TLK7
Locus tag LVJ91_RS10205 Protein ID WP_003409778.1
Coordinates 2199391..2199720 (-) Length 110 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TLK8
Locus tag LVJ91_RS10210 Protein ID WP_003409780.1
Coordinates 2199717..2200094 (-) Length 126 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ91_RS10185 (LVJ91_10200) 2195774..2196844 + 1071 WP_003899091.1 epoxide hydrolase EphB -
LVJ91_RS10190 (LVJ91_10205) 2196841..2197356 + 516 WP_012054301.1 flavin reductase family protein -
LVJ91_RS10195 (LVJ91_10210) 2197353..2198414 + 1062 WP_003899092.1 3,4-dihydroxy-2-butanone-4-phosphate synthase -
LVJ91_RS10200 (LVJ91_10215) 2198411..2199181 + 771 WP_003409775.1 SDR family oxidoreductase -
LVJ91_RS10205 (LVJ91_10220) 2199391..2199720 - 330 WP_003409778.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LVJ91_RS10210 (LVJ91_10225) 2199717..2200094 - 378 WP_003409780.1 type II toxin-antitoxin system antitoxin MazE5 Antitoxin
LVJ91_RS10215 (LVJ91_10230) 2200091..2200681 - 591 WP_003409784.1 SEC-C metal-binding domain-containing protein -
LVJ91_RS10220 (LVJ91_10235) 2200736..2202100 + 1365 WP_012054302.1 HNH endonuclease signature motif containing protein -
LVJ91_RS10225 (LVJ91_10240) 2202255..2202707 - 453 WP_003899095.1 lipoprotein -
LVJ91_RS10230 (LVJ91_10245) 2202771..2203172 + 402 WP_003409869.1 hypothetical protein -
LVJ91_RS10235 (LVJ91_10250) 2203165..2203347 - 183 WP_003409870.1 hypothetical protein -
LVJ91_RS10240 (LVJ91_10255) 2203461..2203811 - 351 WP_003899096.1 hypothetical protein -
LVJ91_RS10245 (LVJ91_10260) 2203822..2204724 - 903 WP_003899097.1 hypothetical protein -
LVJ91_RS10250 (LVJ91_10265) 2204745..2204936 - 192 WP_003409876.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(7-103)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 11787.82 Da        Isoelectric Point: 8.5361

>T228471 WP_003409778.1 NZ_CP089610:c2199720-2199391 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Download         Length: 330 bp

>T228471 NZ_CP123963:2695641-2695748 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGGACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 126 a.a.        Molecular weight: 13560.07 Da        Isoelectric Point: 5.1519

>AT228471 WP_003409780.1 NZ_CP089610:c2200094-2199717 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Download         Length: 378 bp

>AT228471 NZ_CP123963:c2695593-2695527 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TLK7

Antitoxin

Source ID Structure
AlphaFold DB G0TLK8

References