Detailed information of TA system TA228468

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1771495..1772108
• Replicon: chromosome
• Accession: NZ_CP089610
• Organism: Mycobacterium tuberculosis strain I0004290-8

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LVJ91_RS08305
• Protein ID: WP_070894428.1
• Coordinates: 1771704..1772108 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0E8UJ46
• Locus tag: LVJ91_RS08300
• Protein ID: WP_009935474.1
• Coordinates: 1771495..1771698 (+)
• Length: 68 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ91_RS08270 (LVJ91_08280)	1766900..1767280	+	381	WP_003407768.1	fumarate reductase subunit C	-
LVJ91_RS08275 (LVJ91_08285)	1767277..1767654	+	378	WP_003407771.1	fumarate reductase subunit FrdD	-
LVJ91_RS08280 (LVJ91_08290)	1767722..1768330	+	609	WP_003407773.1	TetR/AcrR family transcriptional regulator	-
LVJ91_RS08285 (LVJ91_08295)	1768514..1769662	+	1149	Protein_1634	MMPL family transporter	-
LVJ91_RS08290 (LVJ91_08300)	1769672..1770118	+	447	WP_003407780.1	F420H(2)-dependent quinone reductase	-
LVJ91_RS08295 (LVJ91_08305)	1770153..1771442	+	1290	WP_003407781.1	threonine ammonia-lyase	-
LVJ91_RS08300 (LVJ91_08310)	1771495..1771698	+	204	WP_009935474.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LVJ91_RS08305 (LVJ91_08315)	1771704..1772108	+	405	WP_070894428.1	type II toxin-antitoxin system toxin ribonuclease VapC11	Toxin
LVJ91_RS08310 (LVJ91_08320)	1772125..1773867	-	1743	WP_003407788.1	malto-oligosyltrehalose trehalohydrolase	-
LVJ91_RS08315 (LVJ91_08325)	1773860..1776157	-	2298	WP_003407790.1	malto-oligosyltrehalose synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-44)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14749.90 Da
• Isoelectric Point: 6.2288

>T228468 WP_070894428.1 NZ_CP089610:1771704-1772108 [Mycobacterium tuberculosis]
MILIDTSAWVEYFRATRSIAAVEVRRLLSEEAARIAMCEPIAMEILSGALDDNTHTTLERLVNGLPSLNVDDAIDFRAAA
GIYRAARRAGETVRSINDCLIAALAIRHGARIVHRDADFDVIARITNLQAASFR

Nucleotide

• Length: 405 bp

>T228468 NZ_CP123963:2261081-2261257 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 68 a.a.
• Molecular weight: 7501.48 Da
• Isoelectric Point: 4.3951

>AT228468 WP_009935474.1 NZ_CP089610:1771495-1771698 [Mycobacterium tuberculosis]
MSRTNIDIDDELAAEVMRRFGLTTKRAAVDLALRRLVGSPLSREFLLGLEGVGWEGDLDDLRSDRPD

Nucleotide

• Length: 204 bp

>AT228468 NZ_CP123963:2261303-2261719 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E8UJ46