Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1692995..1693611 Replicon chromosome
Accession NZ_CP089610
Organism Mycobacterium tuberculosis strain I0004290-8

Toxin (Protein)


Gene name mazF Uniprot ID G0TJ74
Locus tag LVJ91_RS07960 Protein ID WP_003407593.1
Coordinates 1693294..1693611 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TJ73
Locus tag LVJ91_RS07955 Protein ID WP_003900349.1
Coordinates 1692995..1693297 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ91_RS07945 (LVJ91_07950) 1688881..1690728 + 1848 WP_003407585.1 methylmalonyl-CoA mutase small subunit -
LVJ91_RS07950 (LVJ91_07955) 1690729..1692981 + 2253 WP_003407587.1 methylmalonyl-CoA mutase -
LVJ91_RS07955 (LVJ91_07960) 1692995..1693297 + 303 WP_003900349.1 type II toxin-antitoxin system antitoxin MazE4 Antitoxin
LVJ91_RS07960 (LVJ91_07965) 1693294..1693611 + 318 WP_003407593.1 type II toxin-antitoxin system toxin endoribonuclease MazF4 Toxin
LVJ91_RS07965 (LVJ91_07970) 1693608..1694612 + 1005 WP_003407596.1 methylmalonyl Co-A mutase-associated GTPase MeaB -
LVJ91_RS07970 (LVJ91_07975) 1694665..1695954 + 1290 WP_003407599.1 serine hydrolase domain-containing protein -
LVJ91_RS07975 (LVJ91_07980) 1696027..1696752 - 726 WP_003898898.1 class I SAM-dependent methyltransferase -
LVJ91_RS07980 (LVJ91_07985) 1696858..1697070 - 213 WP_003898900.1 dodecin family protein -
LVJ91_RS07985 (LVJ91_07990) 1697131..1697529 + 399 WP_003900351.1 hypothetical protein -
LVJ91_RS07990 (LVJ91_07995) 1697574..1698602 + 1029 WP_003407612.1 glycosyltransferase family 2 protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-99)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 11377.97 Da        Isoelectric Point: 7.9950

>T228467 WP_003407593.1 NZ_CP089610:1693294-1693611 [Mycobacterium tuberculosis]
VNAPLRGQVYRCDLGYGAKPWLIVSNNARNRHTADVVAVRLTTTRRTIPTWVAMGPSDPLTGYVNADNIETLGKDELGDY
LGEVTPATMNKINTALATALGLPWP

Download         Length: 318 bp

>T228467 NZ_CP123963:c2167856-2167662 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11173.42 Da        Isoelectric Point: 9.5564

>AT228467 WP_003900349.1 NZ_CP089610:1692995-1693297 [Mycobacterium tuberculosis]
VPFLVALSGIISGVRDHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDEQLDAAYAAAIHDNPAYPYES
EAERSAARARRNARQQRSAQ

Download         Length: 303 bp

>AT228467 NZ_CP123963:2167485-2167663 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5XE3
PDB 5XE2

Antitoxin

Source ID Structure
PDB 5XE3
AlphaFold DB G0TJ73

References