Detailed information of TA system TA228464

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-Phd
• Location: 1393544..1394103
• Replicon: chromosome
• Accession: NZ_CP089610
• Organism: Mycobacterium tuberculosis strain I0004290-8

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G0THS1
• Locus tag: LVJ91_RS06645
• Protein ID: WP_003898789.1
• Coordinates: 1393544..1393837 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G0THS2
• Locus tag: LVJ91_RS06650
• Protein ID: WP_003406322.1
• Coordinates: 1393834..1394103 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ91_RS06620 (LVJ91_06615)	1389137..1389397	+	261	WP_003406302.1	type II toxin-antitoxin system antitoxin VapB33	-
LVJ91_RS06625 (LVJ91_06620)	1389394..1389825	+	432	WP_003406304.1	type II toxin-antitoxin system VapC family toxin	-
LVJ91_RS06630 (LVJ91_06625)	1389848..1391536	-	1689	WP_003910308.1	PE family protein	-
LVJ91_RS06635 (LVJ91_06630)	1391716..1392576	+	861	WP_003900301.1	glycine betaine ABC transporter substrate-binding protein	-
LVJ91_RS06640 (LVJ91_06635)	1392657..1393487	-	831	WP_003898788.1	SDR family NAD(P)-dependent oxidoreductase	-
LVJ91_RS06645 (LVJ91_06640)	1393544..1393837	-	294	WP_003898789.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
LVJ91_RS06650 (LVJ91_06645)	1393834..1394103	-	270	WP_003406322.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LVJ91_RS06655 (LVJ91_06650)	1394216..1397911	-	3696	WP_003898790.1	multifunctional oxoglutarate decarboxylase/oxoglutarate dehydrogenase thiamine pyrophosphate-binding subunit/dihydrolipoyllysine-residue succinyltransferase subunit	-
LVJ91_RS06660 (LVJ91_06655)	1398053..1398841	-	789	WP_003406325.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (9-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11031.57 Da
• Isoelectric Point: 9.6275

>T228464 WP_003898789.1 NZ_CP089610:c1393837-1393544 [Mycobacterium tuberculosis]
VSDDHPYHVAITATAARDLQRLPEKIAAACVEFVFGPLLNNPHRLGKPLRNDLEGLHSARRGDYRVVYAIDDGHHRVEII
HIARRSASYRMNPCRPR

Nucleotide

• Length: 294 bp

>T228464 NZ_CP123963:1804154-1804405 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9766.97 Da
• Isoelectric Point: 4.8615

>AT228464 WP_003406322.1 NZ_CP089610:c1394103-1393834 [Mycobacterium tuberculosis]
MAVVPLGEVRNRLSEYVAEVELTHERITITRHGHPAAVLISADDLASIEETLEVLRTPGASEAIREGLADVAAGRFVSND
EIRNRYTAR

Nucleotide

• Length: 270 bp

>AT228464 NZ_CP123963:1804402-1804656 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: G0THS1

Antitoxin

• AlphaFold DB: G0THS2