Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1235688..1236319 Replicon chromosome
Accession NZ_CP089610
Organism Mycobacterium tuberculosis strain I0004290-8

Toxin (Protein)


Gene name mazF Uniprot ID P9WIH8
Locus tag LVJ91_RS05895 Protein ID WP_003898728.1
Coordinates 1235688..1235999 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TGZ7
Locus tag LVJ91_RS05900 Protein ID WP_003405836.1
Coordinates 1235999..1236319 (-) Length 107 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ91_RS05875 (LVJ91_05875) 1231169..1232593 - 1425 WP_003405805.1 class II fumarate hydratase -
LVJ91_RS05880 (LVJ91_05880) 1232624..1233712 - 1089 WP_003898726.1 class II fructose-bisphosphatase -
LVJ91_RS05885 (LVJ91_05885) 1233768..1234412 + 645 WP_003915494.1 DUF4245 domain-containing protein -
LVJ91_RS05890 (LVJ91_05890) 1234419..1235576 - 1158 WP_003898727.1 AI-2E family transporter -
LVJ91_RS05895 (LVJ91_05895) 1235688..1235999 - 312 WP_003898728.1 type II toxin-antitoxin system toxin endoribonuclease MazF3 Toxin
LVJ91_RS05900 (LVJ91_05900) 1235999..1236319 - 321 WP_003405836.1 type II toxin-antitoxin system antitoxin MazE3 Antitoxin
LVJ91_RS05905 (LVJ91_05905) 1236329..1237865 + 1537 Protein_1163 carboxylesterase/lipase family protein -
LVJ91_RS05910 (LVJ91_05910) 1237872..1238984 - 1113 WP_003405840.1 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase -
LVJ91_RS05915 (LVJ91_05915) 1238994..1239251 - 258 WP_003405844.1 exodeoxyribonuclease VII small subunit -
LVJ91_RS05920 (LVJ91_05920) 1239241..1240488 - 1248 WP_003405846.1 exodeoxyribonuclease VII large subunit -
LVJ91_RS05925 (LVJ91_05925) 1240485..1241123 - 639 WP_003898730.1 lipid droplet-associated protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-85)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 11049.76 Da        Isoelectric Point: 4.9371

>T228461 WP_003898728.1 NZ_CP089610:c1235999-1235688 [Mycobacterium tuberculosis]
MRPIHIAQLDKARPVLILTREVVRPHLTNVTVAPITTTVRGLATEVPVDAVNGLNQPSVVSCDNTQTIPVCDLGRQIGYL
LASQEPALAEAIGNAFDLDWVVA

Download         Length: 312 bp

>T228461 NZ_CP123963:769271-769648 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAGCTTATTGCAATTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCAGGCATGCC
AGATTCGGGAAGAGCTGAGGCTATTATCGGGAGAGTCCAGGCCAGAGTTGCATATGAAGAGATTACCGACCTTTTCGAAG
TCTCCGCAACTTACCTGGTGGCTACTGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
TTGTTATTTCTGCGCCGTAACGGTGTGCAGGTATATGATTCACCAGAACTGGCAGAGCTTACTGTGGGGGCTGCGACCGG
AGAGGTATCCGTATCTACTGTCGTTGCCACGTTACGTAGATTGTATGGTACGGCGTAG

Antitoxin


Download         Length: 107 a.a.        Molecular weight: 11394.83 Da        Isoelectric Point: 5.1236

>AT228461 WP_003405836.1 NZ_CP089610:c1236319-1235999 [Mycobacterium tuberculosis]
MYLPWGVVLAGGANGFGAGAYQTGTICEVSTQIAVRLPDEIVAFIDDEVRGQHARSRAAVVLRALERERRRRLAERDAEI
LATNTSATGDLDTLAGHCARTALDID

Download         Length: 321 bp

>AT228461 NZ_CP123963:769050-769271 [Escherichia coli]
ATGCAATCTATTAACTTCCGTACCGCACGCAGCAATCTTACCGAAGTGCTCAACAATGTTGAAGCCGGAGAAGAGGTTGA
AATCACCCGTAGAGGCCGTGAGCCAGCTGTAATTGTCAGCAAGGCCACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTCGCATCCCTGTTTGACACTCTGGACACCACCAATAAGAAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CCA
PDB 5UCT

Antitoxin

Source ID Structure
AlphaFold DB G0TGZ7

References