Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1029264..1030237 Replicon chromosome
Accession NZ_CP089610
Organism Mycobacterium tuberculosis strain I0004290-8

Toxin (Protein)


Gene name tacT Uniprot ID O05911
Locus tag LVJ91_RS04880 Protein ID WP_003404756.1
Coordinates 1029737..1030237 (+) Length 167 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID O05910
Locus tag LVJ91_RS04875 Protein ID WP_003898647.1
Coordinates 1029264..1029740 (+) Length 159 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ91_RS04855 (LVJ91_04850) 1025111..1026382 - 1272 WP_003404747.1 PPE family protein -
LVJ91_RS04860 (LVJ91_04855) 1026397..1026696 - 300 WP_003404748.1 PE family protein -
LVJ91_RS04865 (LVJ91_04860) 1027140..1028921 + 1782 WP_003404749.1 BCCT family transporter -
LVJ91_RS04870 (LVJ91_04865) 1028921..1029175 + 255 WP_003898646.1 hypothetical protein -
LVJ91_RS04875 (LVJ91_04870) 1029264..1029740 + 477 WP_003898647.1 DUF1778 domain-containing protein Antitoxin
LVJ91_RS04880 (LVJ91_04875) 1029737..1030237 + 501 WP_003404756.1 GNAT family N-acetyltransferase Toxin
LVJ91_RS04890 (LVJ91_04885) 1030550..1031869 - 1320 WP_003404757.1 IS256-like element IS1554 family transposase -
LVJ91_RS04895 (LVJ91_04890) 1032157..1032738 + 582 WP_003404760.1 IS607-like element IS1535 family transposase -
LVJ91_RS04900 (LVJ91_04895) 1032738..1034390 + 1653 WP_003404761.1 IS607 family element RNA-guided endonuclease TnpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(38-143)

Antitoxin

Vibrio phage ICP1, Orf50

(74-150)


Sequences

Toxin        


Download         Length: 167 a.a.        Molecular weight: 18048.55 Da        Isoelectric Point: 7.8659

>T228459 WP_003404756.1 NZ_CP089610:1029737-1030237 [Mycobacterium tuberculosis]
VSGYSAPRRISDADDVTSFSSGEPSLDDYLRKRALANHVQGGSRCFVTCRDGRVVGFYALASGSVAHADAPGRVRRNMPD
PVPVILLSRLAVDRKEQGRGLGSHLLRDAIGRCVQAADSIGLRAILVHALHDEARAFYVHFDFEISPTDPLHLMLLMKDA
RALIGD

Download         Length: 501 bp

>T228459 NZ_CP123963:653896-654210 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 159 a.a.        Molecular weight: 17387.98 Da        Isoelectric Point: 11.2467

>AT228459 WP_003898647.1 NZ_CP089610:1029264-1029740 [Mycobacterium tuberculosis]
MHRAGAAVTANVWCRAGGIRMAPRPVIPVATQQRLRRQADRQSLGSSGLPALNCTPIRHTIDVMATKPERKTERLAARLT
PEQDALIRRAAEAEGTDLTNFTVTAALAHARDVLADRRLFVLTDAAWTEFLAALDRPVSHKPRLEKLFAARSIFDTEG

Download         Length: 477 bp

>AT228459 NZ_CP123963:654207-654623 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BSZ2

Antitoxin

Source ID Structure
AlphaFold DB A5U0V8

References