Detailed information of TA system TA228454

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 758372..758912
• Replicon: chromosome
• Accession: NZ_CP089610
• Organism: Mycobacterium tuberculosis strain I0004290-8

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: P9WII0
• Locus tag: LVJ91_RS03485
• Protein ID: WP_003403376.1
• Coordinates: 758372..758680 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TQE0
• Locus tag: LVJ91_RS03490
• Protein ID: WP_003403381.1
• Coordinates: 758667..758912 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ91_RS03460 (LVJ91_03455)	753687..755192	+	1506	WP_003403360.1	carotenoid cleavage oxygenase	-
LVJ91_RS03465 (LVJ91_03460)	755273..756283	+	1011	WP_009937374.1	ABC transporter ATP-binding protein	-
LVJ91_RS03470 (LVJ91_03465)	756671..757054	-	384	WP_003403365.1	ribonuclease VapC6	-
LVJ91_RS03475 (LVJ91_03470)	757149..757304	-	156	WP_003403368.1	type II toxin-antitoxin system VapB family antitoxin	-
LVJ91_RS03480 (LVJ91_03475)	757380..758096	-	717	WP_003403371.1	CPBP family intramembrane metalloprotease	-
LVJ91_RS03485 (LVJ91_03480)	758372..758680	-	309	WP_003403376.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LVJ91_RS03490 (LVJ91_03485)	758667..758912	-	246	WP_003403381.1	ribbon-helix-helix protein, CopG family	Antitoxin
LVJ91_RS03495 (LVJ91_03490)	759022..759459	-	438	WP_003403386.1	type II toxin-antitoxin system VapC family toxin	-
LVJ91_RS03500 (LVJ91_03495)	759456..759710	-	255	WP_003911263.1	antitoxin VapB7	-
LVJ91_RS03505 (LVJ91_03500)	759824..762187	+	2364	WP_003898547.1	arylsulfatase AtsD	-
LVJ91_RS03510 (LVJ91_03505)	762250..762576	+	327	WP_003403401.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
LVJ91_RS03515 (LVJ91_03510)	762488..762826	+	339	WP_003403405.1	PIN domain-containing protein	-
LVJ91_RS03520 (LVJ91_03515)	762823..762996	+	174	WP_003898549.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-101)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11320.10 Da
• Isoelectric Point: 11.6554

>T228454 WP_003403376.1 NZ_CP089610:c758680-758372 [Mycobacterium tuberculosis]
MRRGELWFAATPGGDRPVLVLTRDPVADRIGAVVVVALTRTRRGLVSELELTAVENRVPSDCVVNFDNIHTLPRTAFRRR
ITRLSPARLHEACQTLRASTGC

Nucleotide

• Length: 309 bp

>T228454 NZ_CP123958:1842140-1842436 [Mannheimia haemolytica]
ATGTATATAGATTTGCAATATGCTGTGCCGTTATTGTTTGAATACGACCCGAACAAAAGCCAAATTAATTTAGCCAAATA
TGGTATTGATTTTGAACAGGCAAAGTTGCTTTGGGAAGATGAACGAAAAGTTGTACTGGAGGCTTGCATTGAACCCGAAT
TACGCTATTTCCTGATTGGCAAGATTTATGATAAATATTGGACAGCGTTTTTTACACCAAGAAATGATGTTATTCGTTTA
ATTTCAGTTAGACGTTCCCGTAAAAAAGAGATCTCATACTATGAGAAATACCATTAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9169.01 Da
• Isoelectric Point: 4.2070

>AT228454 WP_003403381.1 NZ_CP089610:c758912-758667 [Mycobacterium tuberculosis]
MLSFRADDHDVDLADAWARRLHIGRSELLRDALRRHLAALAADQDVQAYTERPLTDDENALAEIADWGPAEDWADWADAA
R

Nucleotide

• Length: 246 bp

>AT228454 NZ_CP123958:1842420-1842662 [Mannheimia haemolytica]
ATGAGAAATACCATTAGTGCCGAAGAATTAGAACGTATCTTCGATGAAGGCAAAGAAGATATTTTACAATACTGCGATCT
TTCTACCGCTCATCGCCCGGGGCAAATGAAAAGGGTGAATGTTGATTTCCCAAATTGGATGGTAGAAGCGTTAGATAAGC
AAGCCAGACATTTAGGTGTGCCTCGCCAAGCCTTGATTAAAATGTGGATTGCGGAAAAATTACCGGTTCAATCACCTAGG
TAA

Structures

Toxin

• AlphaFold DB: A0A7U4BSE5

Antitoxin

• AlphaFold DB: G0TQE0