Detailed information of TA system TA228449

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 706401..707044
• Replicon: chromosome
• Accession: NZ_CP089610
• Organism: Mycobacterium tuberculosis strain I0004290-8

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P67239
• Locus tag: LVJ91_RS03200
• Protein ID: WP_003403187.1
• Coordinates: 706643..707044 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ91
• Locus tag: LVJ91_RS03195
• Protein ID: WP_003403184.1
• Coordinates: 706401..706646 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ91_RS03160 (LVJ91_03155)	701681..702211	-	531	WP_003917320.1	two component system sensor kinase HK2	-
LVJ91_RS03165 (LVJ91_03160)	702195..702890	-	696	WP_003918099.1	two-component system response regulator TcrA	-
LVJ91_RS03170 (LVJ91_03165)	703013..703324	+	312	WP_003403164.1	hypothetical protein	-
LVJ91_RS03175 (LVJ91_03170)	703396..704346	+	951	WP_003900184.1	DUF1259 domain-containing protein	-
LVJ91_RS03180 (LVJ91_03175)	704587..705171	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
LVJ91_RS03185 (LVJ91_03180)	705173..705883	+	711	Protein_631	IS607 family element RNA-guided endonuclease TnpB	-
LVJ91_RS03190 (LVJ91_03185)	705886..706356	+	471	WP_003898523.1	hypothetical protein	-
LVJ91_RS03195 (LVJ91_03190)	706401..706646	+	246	WP_003403184.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LVJ91_RS03200 (LVJ91_03195)	706643..707044	+	402	WP_003403187.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ91_RS03205 (LVJ91_03200)	707035..707214	+	180	Protein_635	hypothetical protein	-
LVJ91_RS03210 (LVJ91_03205)	707632..707829	-	198	WP_003403191.1	hypothetical protein	-
LVJ91_RS03215 (LVJ91_03210)	707909..709066	-	1158	WP_003898524.1	hypothetical protein	-
LVJ91_RS03220 (LVJ91_03215)	709118..709339	-	222	WP_003898525.1	DUF4926 domain-containing protein	-
LVJ91_RS03225 (LVJ91_03220)	709481..710086	+	606	WP_003898526.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-80)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14501.21 Da
• Isoelectric Point: 4.4463

>T228449 WP_003403187.1 NZ_CP089610:706643-707044 [Mycobacterium tuberculosis]
VIVDTSAIIAILRDEDDAAAYADALANADVRRLSAASYLECGIVLDSQRDPVISRALDELIEEAEFVVEPVTERQARLAR
AAYADFGRGSGHPAGLNFGDCLSYALAIDRREPLLWKGNDFGHTGVQRALDRR

Nucleotide

• Length: 402 bp

>T228449 NZ_CP123958:217732-217956 [Mannheimia haemolytica]
ATGGTAGTAAAAATCCTAAAGCGGAAATCTCAAAAGACACTACAACAAATCTACACCCAGCCTATTTCTGCCAATATTAA
ATGGACAAATATAGAAGCTCTTTTTCTAGAGTTAGGAGCAGAAATAGAAGAACGAGAAGGGAGCAGAATTGCAGTTGTTT
TATTTCAACAGGTCAGAGTATTTCATCGCCCACACCCGAATCCTGATACAGATAAGGGGCGGTAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8813.12 Da
• Isoelectric Point: 10.3486

>AT228449 WP_003403184.1 NZ_CP089610:706401-706646 [Mycobacterium tuberculosis]
MALNIKDPSVHQAVKQIAKITGESQARAVATAVNERLARLRSDDLAARLLAIGHKTASRMSPEAKRLDHDALLYDERGLP
A

Nucleotide

• Length: 246 bp

>AT228449 NZ_CP123958:217999-218328 [Mannheimia haemolytica]
ATGCGTAATATTATGGATATTAATGGTTATAAAGCTGTCATTGCTTACGATCCCGAAACAGAATTATTTCGAGGTGAATT
TATCGGATTAAATGGTGGAGCCGATTTTTATGCCGATAATGTCATTCAATTAAAAAAAGAAGGTGAACTAAGTTTGAATA
TCTTTTTAGAACTTTGTAGAGAAAAAGGTATTGAGCCGTTCAAACAATATTCCGGAAAGTTTAATGTTCGCCTTTCGCCT
GAATTACATAAAGCTGCGGTAATTGCAGCAACTGCTGAAAATTTAAGCTTAAATGAATGGATTAACCAAACGTTAGAAAA
GAGCGTGTAG

Structures

Toxin

• AlphaFold DB: A0A7U4BSD6

Antitoxin

• AlphaFold DB: G0TQ91