Detailed information of TA system TA228448

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 700311..700957
• Replicon: chromosome
• Accession: NZ_CP089610
• Organism: Mycobacterium tuberculosis strain I0004290-8

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ88
• Locus tag: LVJ91_RS03145
• Protein ID: WP_003403137.1
• Coordinates: 700311..700724 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ89
• Locus tag: LVJ91_RS03150
• Protein ID: WP_003403139.1
• Coordinates: 700721..700957 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ91_RS03125 (LVJ91_03120)	696394..697944	+	1551	WP_003403119.1	MlaD family protein	-
LVJ91_RS03130 (LVJ91_03125)	697996..698388	-	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	-
LVJ91_RS03135 (LVJ91_03130)	698385..698642	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	-
LVJ91_RS03140 (LVJ91_03135)	698825..700060	-	1236	WP_003403128.1	ATP-binding protein	-
LVJ91_RS03145 (LVJ91_03140)	700311..700724	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ91_RS03150 (LVJ91_03145)	700721..700957	-	237	WP_003403139.1	type II toxin-antitoxin system antitoxin VapB27	Antitoxin
LVJ91_RS03155 (LVJ91_03150)	701061..701567	-	507	WP_003900973.1	two component system sensor kinase HK1	-
LVJ91_RS03160 (LVJ91_03155)	701681..702211	-	531	WP_003917320.1	two component system sensor kinase HK2	-
LVJ91_RS03165 (LVJ91_03160)	702195..702890	-	696	WP_003918099.1	two-component system response regulator TcrA	-
LVJ91_RS03170 (LVJ91_03165)	703013..703324	+	312	WP_003403164.1	hypothetical protein	-
LVJ91_RS03175 (LVJ91_03170)	703396..704346	+	951	WP_003900184.1	DUF1259 domain-containing protein	-
LVJ91_RS03180 (LVJ91_03175)	704587..705171	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
LVJ91_RS03185 (LVJ91_03180)	705173..705883	+	711	Protein_631	IS607 family element RNA-guided endonuclease TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-126)

Antitoxin

• SpoVT-AbrB domain (3-43)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14786.14 Da
• Isoelectric Point: 8.1405

>T228448 WP_003403137.1 NZ_CP089610:c700724-700311 [Mycobacterium tuberculosis]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Nucleotide

• Length: 414 bp

>T228448 NZ_CP123958:195591-195860 [Mannheimia haemolytica]
ATGCTACAAATTTCGCCGACAAACGCATATAAAAGAGACTTTAAAAAGATTGCAGCCGAATTAGTCGGTAGTTCGGAATA
TGTGGAAGTAATGTATTGCCTAATAAACCAATTACCATTGGCGGAAAAATATAGAGATCACCCACTACAAGGTGAATGGC
AAGGCTTTAGAGATTGCCATATTAAGCCTGATTTAGTGTTGATTTACGCTGTTGAAGATAATCTGCTCCGCCTTGTTCGT
TTAGGATCGCACGCTGAATTATTTGGATAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8212.41 Da
• Isoelectric Point: 5.0351

>AT228448 WP_003403139.1 NZ_CP089610:c700957-700721 [Mycobacterium tuberculosis]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Nucleotide

• Length: 237 bp

>AT228448 NZ_CP123958:195321-195587 [Mannheimia haemolytica]
ATGGCGACTATCAATGATGCTTTCAGTTTTAGAACAAATACCGAAATAAAAAATACCGCATTTGATGTAATTAAAAACTA
TGGAATGACACCCTCTCAGGTGTTTAATATGTTTTTAACCGAGATTGCAAAAACGAAAACTATTCCGTTAAGTTTGAATT
ATCAACCCAATCTTGAAACAAAATTGGCAATGCAAGAAGCAAAATCAGGTAAAAATGAAGTTTATGCCTCACTTGAAGCA
TTTCATAAAGCAATGTTAGCGGAGTAA

Structures

Toxin

• AlphaFold DB: G0TQ88

Antitoxin

• AlphaFold DB: G0TQ89