Detailed information of TA system TA228445

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-CcdA
• Location: 643385..644061
• Replicon: chromosome
• Accession: NZ_CP089610
• Organism: Mycobacterium tuberculosis strain I0004290-8

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TPQ9
• Locus tag: LVJ91_RS02895
• Protein ID: WP_003898500.1
• Coordinates: 643385..643798 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TPR0
• Locus tag: LVJ91_RS02900
• Protein ID: WP_003402915.1
• Coordinates: 643795..644061 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ91_RS02865 (LVJ91_02860)	638730..639032	-	303	WP_003402896.1	DUF3349 domain-containing protein	-
LVJ91_RS02870 (LVJ91_02865)	639092..639370	-	279	WP_003402901.1	hypothetical protein	-
LVJ91_RS02875 (LVJ91_02870)	639367..640620	-	1254	WP_003402904.1	inorganic phosphate transporter	-
LVJ91_RS02880 (LVJ91_02875)	640740..641126	-	387	WP_003402907.1	VOC family protein	-
LVJ91_RS02885 (LVJ91_02880)	641189..642073	-	885	WP_003402909.1	SDR family oxidoreductase	-
LVJ91_RS02890 (LVJ91_02885)	642169..643071	-	903	WP_003915439.1	1,4-dihydroxy-2-naphthoyl-CoA synthase	-
LVJ91_RS02895 (LVJ91_02890)	643385..643798	-	414	WP_003898500.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ91_RS02900 (LVJ91_02895)	643795..644061	-	267	WP_003402915.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
LVJ91_RS02905 (LVJ91_02900)	644253..645968	-	1716	WP_003402917.1	fatty-acid--CoA ligase FadD8	-
LVJ91_RS02910 (LVJ91_02905)	646046..647650	+	1605	WP_003402920.1	amidohydrolase	-
LVJ91_RS02915 (LVJ91_02910)	647647..648627	+	981	WP_003402923.1	o-succinylbenzoate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (13-129)

Antitoxin

• Post-segregation antitoxin CcdA (19-61)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14667.78 Da
• Isoelectric Point: 7.3603

>T228445 WP_003898500.1 NZ_CP089610:c643798-643385 [Mycobacterium tuberculosis]
VRASPTSPPEQVVVDASAMVDLLARTSDRCSAVRARLARTAMHAPAHFDAEVLSALGRMQRAGALTVAYVDAALEELRQV
PVTRHGLSSLLAGAWSRRDTLRLTDALYVELAETAGLVLLTTDERLARAWPSAHAIG

Nucleotide

• Length: 414 bp

>T228445 NZ_CP123957:c4076527-4076345 [Pseudomonas marginalis]
ATGAGAAGTCGAGAGGTGATTGACCTGCTGGTTGCGGATGGTTGGTTTGAGGTGGCAGTGAAGGGCAGTCATCATCAGTT
CAAGCACCCCATGAAGCGGGGAAGAGTCACCGTCCCTCATCCCAAGTCAGAGATTGCCAAGGGCACTCTGCACAGTATTT
TCAAATCAGCCGGTCTCAAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9543.79 Da
• Isoelectric Point: 4.5994

>AT228445 WP_003402915.1 NZ_CP089610:c644061-643795 [Mycobacterium tuberculosis]
MLSRRTKTIVVCTLVCMARLNVYVPDELAERARARGLNVSALTQAAISAELENSATDAWLEGLEPRSTGARHDDVLGAID
AARDEFEA

Nucleotide

• Length: 267 bp

>AT228445 NZ_CP123957:c4076290-4075889 [Pseudomonas marginalis]
ATGAAATTCCCAGTGGTAGTACACAAAGATGCTGATTCGGATTACAGCGTGACTGTCCCTGATGTGCCGGGGTGTTTCTC
GGCGGGCGGGTCTTTTTCCGAAGCGCTGGACAATGTACGTGAGGCATTGGCTCTGCACCTCGAAGGCCTGGTTGCCGATC
AGGAACAGCTTCCTCAGGCGCTGGAGGTTGATGCCCATTTGGGTAACCCGGATTTTGCCGGTGGTGTCTGGGCGATAGTC
GACTTTGACCTCACGCCTTATCTGGGGAAATCAGTGCGGTTCAATGCCTCTCTGCCGGAACACCTCTTGCAGCGGATTGA
CGAGCGTGTGCAAAAGGATCATCGCTATGCATCGCGGTCAGGATTTTTGGCGACGGCAGCGCTGCGTGAGCTCTCTGCGT
AA

Structures

Toxin

• AlphaFold DB: G0TPQ9

Antitoxin

• AlphaFold DB: G0TPR0