Detailed information of TA system TA228444

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-ParD
• Location: 447317..448328
• Replicon: chromosome
• Accession: NZ_CP089610
• Organism: Mycobacterium tuberculosis strain I0004290-8

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: A0A655I6S7
• Locus tag: LVJ91_RS01935
• Protein ID: WP_003906352.1
• Coordinates: 447317..447910 (-)
• Length: 198 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: O53702
• Locus tag: LVJ91_RS01940
• Protein ID: WP_003401860.1
• Coordinates: 447939..448328 (-)
• Length: 130 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ91_RS01915 (LVJ91_01915)	442966..444348	+	1383	WP_003898415.1	magnesium transporter	-
LVJ91_RS01920 (LVJ91_01920)	444360..445394	-	1035	WP_003401856.1	class II fructose-bisphosphate aldolase	-
LVJ91_RS01925 (LVJ91_01925)	445490..446173	+	684	WP_003401857.1	VTT domain-containing protein	-
LVJ91_RS01930 (LVJ91_01930)	446162..447292	-	1131	WP_003401858.1	glycoside hydrolase family 76 protein	-
LVJ91_RS01935 (LVJ91_01935)	447317..447910	-	594	WP_003906352.1	zeta toxin family protein	Toxin
LVJ91_RS01940 (LVJ91_01940)	447939..448328	-	390	WP_003401860.1	hypothetical protein	Antitoxin
LVJ91_RS01945 (LVJ91_01945)	448409..449620	-	1212	WP_003906353.1	VWA domain-containing protein	-
LVJ91_RS01950 (LVJ91_01950)	449626..450228	-	603	WP_003898417.1	SRPBCC family protein	-
LVJ91_RS01955 (LVJ91_01955)	450242..451138	-	897	WP_003898418.1	MoxR family ATPase	-
LVJ91_RS01960 (LVJ91_01960)	451135..451728	-	594	WP_003401864.1	NTP transferase domain-containing protein	-
LVJ91_RS01965 (LVJ91_01965)	451725..452480	-	756	WP_003401865.1	XdhC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (4-156)

Antitoxin

• ParD-like antitoxin of type II bacterial toxin-antitoxin system (6-106)

Sequences

Toxin

Protein

• Length: 198 a.a.
• Molecular weight: 21358.60 Da
• Isoelectric Point: 10.6337

>T228444 WP_003906352.1 NZ_CP089610:c447910-447317 [Mycobacterium tuberculosis]
VKRLDLVAGPNGAGKSTFVALTLAPLLPGIVFVNADEIAKQRWPDDPTSHAYQAAQVAADTRARLIDLGRPFIAETVFSH
PSKLELIRTARTAGYTVVLHVLVIPEGLAVERVRHRVAAGGHDVPETKIRERHRRLAELVAQAITLADGATVYDKSRLAG
PRIVAQFSGGGIIGRACWPSWTPPPLMSRWSNRPETA

Nucleotide

• Length: 594 bp

>T228444 NZ_CP123957:2411443-2411847 [Pseudomonas marginalis]
ATGATTAAGTACATGCTTGACACCAACATCGTGATCTACATCATCAAGCGTCGGCCCATCGAAATACTGGAAAAATTCAA
CGCGAACGTAGGGCGTATGGTGATCTCTTCAATTACCCTCGCAGAGCTGATGCATGGAGCAGAAAAGAGTCAGCTCGTTG
AGAAAAATACGCGAGCCGTGGAAGACTTTTCTTCCCGGCTCGACGTCCTTAACTACGACGACAAGGCCGCCTTTCACTAC
GGCTCGATTCGTTCGGATCTGGAGAAAAAGGGCACGCCTATTGGTGTAAATGACCTCCATATCGCCGGGCACGCTAGAAG
CGCAGGCTTAGTGTTGGTGACTAACAATGAATGTGAGTTCAAGCGCGTGAATGGCTTGATTGTGGAGAACTGGGTAGCGG
CTTAA

Antitoxin

Protein

• Length: 130 a.a.
• Molecular weight: 13648.21 Da
• Isoelectric Point: 6.0829

>AT228444 WP_003401860.1 NZ_CP089610:c448328-447939 [Mycobacterium tuberculosis]
VPKAVDRVTRVAADLVDSAAAEGARQSRSAKQQLDHWARVGRAVSNQHTASRRRVEAALAGHLPMTDLTLEEGVVFNAEI
SAAIEERLSRTNYGDVLAAQGITTVALNDAGDIVEHRPDGTSVVLAATP

Nucleotide

• Length: 390 bp

>AT228444 NZ_CP123957:2411211-2411450 [Pseudomonas marginalis]
ATGTCTTCTGGAACGATCTTCACTAACAATCGAACGCAAAATATTCGTATCCCTGCTGACATGCGCTTTCCTGCGGGGGT
GAAAACTGTCGAGGTCCGCGCTGTGGGACGCGAGCTGATCATTTCGCCGGTTGAAAACACTTGGGACAGCTTTTTCCTTG
CAGGCGAATCGGTATCTGATGATTTTATGGAAGAGCGCGCCGATCAAACCCAGCAAGAACGAGAGTCTTTTGATGATTAA

Structures

Toxin

• AlphaFold DB: A0A655I6S7

Antitoxin

• AlphaFold DB: A0A7U4BRT0