Detailed information of TA system TA228440

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 302420..302985
• Replicon: plasmid unnamed
• Accession: NZ_CP089607
• Organism: Rhodococcus qingshengii strain J1-2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: C3JNM9
• Locus tag: LRQ08_RS30795
• Protein ID: WP_003943396.1
• Coordinates: 302629..302985 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: LRQ08_RS30790
• Protein ID: WP_039972906.1
• Coordinates: 302420..302632 (+)
• Length: 71 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRQ08_RS30765 (LRQ08_30675)	298575..299282	-	708	WP_256521212.1	SigB/SigF/SigG family RNA polymerase sigma factor	-
LRQ08_RS30770 (LRQ08_30680)	299348..299782	-	435	WP_256520746.1	ATP-binding protein	-
LRQ08_RS30775 (LRQ08_30685)	299916..300542	-	627	WP_256521213.1	PAS and ANTAR domain-containing protein	-
LRQ08_RS30780 (LRQ08_30690)	300907..301308	+	402	WP_256520747.1	VOC family protein	-
LRQ08_RS30785 (LRQ08_30695)	301765..302013	+	249	WP_256520748.1	hypothetical protein	-
LRQ08_RS30790 (LRQ08_30700)	302420..302632	+	213	WP_039972906.1	ribbon-helix-helix domain-containing protein	Antitoxin
LRQ08_RS30795 (LRQ08_30705)	302629..302985	+	357	WP_003943396.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LRQ08_RS30800 (LRQ08_30710)	303409..305193	-	1785	WP_081559348.1	Hsp70 family protein	-
LRQ08_RS30805 (LRQ08_30715)	305260..307023	-	1764	WP_256520749.1	Hsp70 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..595472	595472

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-117)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-39)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12844.58 Da
• Isoelectric Point: 6.8817

>T228440 WP_003943396.1 NZ_CP089607:302629-302985 [Rhodococcus qingshengii]
MIRRAHIHWVDLDPAQGSEAAKRRPAVVVSNDRANATAENLGRGVITVVPLTTNTAHVFPFQVIVESDESGLARASKAQA
EQVRSVSVRRLDPEPVGKVGMETFAALEDALRLHLDLQ

Nucleotide

• Length: 357 bp

>T228440 NZ_CP123953:5662613-5663137 [Pseudomonas aeruginosa]
ATGAAAATCATCGGAGTGCAGGACCTCCCCTGCTACCGAATGCACACTCCCAAGTGGTCCGTTGCGCCTACATCTGGAGC
TGGAGCTGCAACCCAGGGTGGCCGGGTTAACCGAAAGGGGCTAGAGGCTCTATACCTCTCGCTTGAGTCGGCAACGGCCA
TTGCTGAGTTTCAGCAAGGAAGCGCATTCCTTCCACCAGGTCTGCTGGTCAGCTATTCCCTCAGTCTGGACCGAGTGATC
GACTTCACCGGCGGCTATGACGATACCTGGGCCCCGATCTGGCAAGACTTTTTCTGCGACTGGAGAAAGATTCGCTTCAA
CGACGGCGTTGAACCGCCGAGCTGGATTGTCGGAGATCTGGCTATCGAGGCTGACTGTGCTGGGATTCTGTTCGAGTCCG
TTGCGAACCCTGGAGGTCGGAACCTGGTGCTTTTCACCGATCAGTTGCCGGTGCATGGGAATATTGTGGTAAACGATCCT
CGCGGGGATCTCCCTACCGACCAGTCATCTTGGACGCGCCCGTAG

Antitoxin

Protein

• Length: 71 a.a.
• Molecular weight: 7682.43 Da
• Isoelectric Point: 4.1824

>AT228440 WP_039972906.1 NZ_CP089607:302420-302632 [Rhodococcus qingshengii]
MTTKFTISIPDEDAHLLDEYVRDHGLRGRSAGIRAALRLLSTSGLERDYAAAFAEGDVDEVWDAVTADTE

Nucleotide

• Length: 213 bp

>AT228440 NZ_CP123953:5662221-5662616 [Pseudomonas aeruginosa]
ATGGCCAGCAAAGCCGTATTAGAGCTTCGCCCCACCAACTTCGCGGGCTTGATGGACGCTCTCAAAGAGGTGAACCGTGT
GATGTTCTCTCCTGAGCGCTTCCTCAAGGTTTTTTCGATGGACCAGCAGACCTTGGCGCTCCGGGCCCATGTCCATCGCA
ACACCGTGCGCAACGCGCCTGAATCGGAAAAGGTCCAAGCCTACATTCGTGATTCAGTGAAGGTTCTGCGTGCAGTGACC
GACATGGGCACGGACGTGACGAATGCCATCTTCTGGTTTAAGAACGAGCCTCTTTCCACGTTCAACTACAAGACCGCGGA
GGAAGTGGTCAGCGAAGGAAAAACCGAGCAGCTGATCGCTTTCCTTCAATCCTGGGAAGCTGGTGCGCAAGGATGA

Structures

Toxin

• AlphaFold DB: A0A1F2PUJ5