Detailed information of TA system TA228439

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 228700..229235
• Replicon: plasmid unnamed
• Accession: NZ_CP089607
• Organism: Rhodococcus qingshengii strain J1-2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A7I8BDH8
• Locus tag: LRQ08_RS30400
• Protein ID: WP_042445814.1
• Coordinates: 228700..228999 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: LRQ08_RS30405
• Protein ID: WP_064688354.1
• Coordinates: 228999..229235 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRQ08_RS30385 (LRQ08_30300)	224826..225896	+	1071	WP_256521139.1	PDDEXK nuclease domain-containing protein	-
LRQ08_RS30390 (LRQ08_30305)	226024..226821	-	798	WP_054186839.1	hypothetical protein	-
LRQ08_RS30395 (LRQ08_30310)	226821..228671	-	1851	WP_256521140.1	hypothetical protein	-
LRQ08_RS30400 (LRQ08_30315)	228700..228999	-	300	WP_042445814.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LRQ08_RS30405 (LRQ08_30320)	228999..229235	-	237	WP_064688354.1	YlcI/YnfO family protein	Antitoxin
LRQ08_RS30410 (LRQ08_30325)	229608..230279	-	672	WP_256521141.1	TetR/AcrR family transcriptional regulator	-
LRQ08_RS30415 (LRQ08_30330)	230344..231312	-	969	WP_256521142.1	DUF2637 domain-containing protein	-
LRQ08_RS30420 (LRQ08_30335)	231934..232248	-	315	WP_256521143.1	hypothetical protein	-
LRQ08_RS30425 (LRQ08_30340)	232245..233027	-	783	WP_029256883.1	ParA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..595472	595472

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (11-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 10441.94 Da
• Isoelectric Point: 4.3831

>T228439 WP_042445814.1 NZ_CP089607:c228999-228700 [Rhodococcus qingshengii]
MRPIHEADLDTVRPVLVLTREVVRPHLGTVTVAPITDEIRGLSTEVSVSAANGLSADAVVSCDHITTIPVSALGNVIGYL
LPAQEESLSIAVRAAFDLE

Nucleotide

• Length: 300 bp

>T228439 NZ_CP123953:c5218031-5217651 [Pseudomonas aeruginosa]
GTGAGCGGCCGTTGGCACCAGGCCGGCAGACCGGTCGTCTACACCGCGACCAGCCCGCCGGGAGCAATGCTCGAGGTGCT
TGTTCATCTGGAAATTGATCCAGAGGATTTTCCGGCGACGATGCAGCTTCTCCGTATCGAAGTGCCTGACTCTGTATCAC
AAGCCCAGATGCCCACTTTGCCGGCTGGTTGGTCGGCAGAGACCGAGCTCACCAGAGGCATTGGTAACCAGTTCCTTGAT
GAGTGCAACGCACTGCTACTGCCAGTGCCCAGCGCTATCATACCGAGCACGGTGAACTACCTGTTCAACCCTCGGCACCC
AGAAGCACAGAGCGCGACTATTCAGGTTGAGCTTTTCACCCCTGATTCGAGGTTGTTCTAG

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8642.63 Da
• Isoelectric Point: 6.4809

>AT228439 WP_064688354.1 NZ_CP089607:c229235-228999 [Rhodococcus qingshengii]
MSTQIAVRLPDEIVAFLDDEIREHRATSRASVVLRALERERRRQIAARDAEILANATSEHDLSSLAHHAANPQSTGLS

Nucleotide

• Length: 237 bp

>AT228439 NZ_CP123953:c5218534-5218085 [Pseudomonas aeruginosa]
ATGCTTGCTGAAGTCCTACGCGACCACGGGTATTCAGCTTACCGTGCGCGCTTACAAATACTGCTGGAGATCCCAGAGTC
TGCCAGCGACTTCGAGATTCACACCAGAATCAGCCATGGCTTTTCCGCAGCGCGGTTAATGAGGCTCACTGAAGAGGGGG
TGGTTACCCCGCTGGAGCGAGACCAGATTATCCCGCTGCGCACCCTGAAAAACCGGATCGAGCGCGATCAGCCCCTCACT
GTCGAGGAAAGCGACCGGCTCTTCCGATCGGCGCACATCACCGCGATGGCTGAGGCTATTTTTGGGGACGCCGACAAGGC
CAAGCGATGGCTCTCCAAGCCCAAGGAACGATTCTCTGGGTTGACGCCCATGCAACTGCTCACAACTCAGCAAGGCACAA
GCCAGGTTGAAGAGATGCTGCTGCAAATCGCCGAAGGCTTCGCTCTGTGA

Structures

Toxin

• AlphaFold DB: A0A7I8BDH8