Detailed information of TA system TA228438

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 109962..110480
• Replicon: plasmid unnamed
• Accession: NZ_CP089607
• Organism: Rhodococcus qingshengii strain J1-2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: LRQ08_RS29880
• Protein ID: WP_256521052.1
• Coordinates: 109962..110264 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: LRQ08_RS29885
• Protein ID: WP_256521053.1
• Coordinates: 110265..110480 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRQ08_RS29860 (LRQ08_29785)	105143..105511	+	369	WP_256521050.1	hypothetical protein	-
LRQ08_RS29865 (LRQ08_29790)	105661..106944	+	1284	WP_075835742.1	IS256 family transposase	-
LRQ08_RS29870 (LRQ08_29795)	107175..107972	-	798	WP_007733683.1	hypothetical protein	-
LRQ08_RS29875 (LRQ08_29800)	107972..109933	-	1962	WP_256521051.1	hypothetical protein	-
LRQ08_RS29880 (LRQ08_29805)	109962..110264	-	303	WP_256521052.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LRQ08_RS29885 (LRQ08_29810)	110265..110480	-	216	WP_256521053.1	hypothetical protein	Antitoxin
LRQ08_RS29890 (LRQ08_29815)	110644..111345	+	702	WP_256521055.1	hypothetical protein	-
LRQ08_RS29895 (LRQ08_29820)	111518..112183	+	666	WP_256521200.1	hypothetical protein	-
LRQ08_RS29900 (LRQ08_29825)	112669..113694	-	1026	WP_256521056.1	hypothetical protein	-
LRQ08_RS29905 (LRQ08_29830)	113798..114442	+	645	WP_256521057.1	TetR/AcrR family transcriptional regulator	-
LRQ08_RS29910 (LRQ08_29835)	115010..115267	-	258	WP_256521058.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..595472	595472

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-95)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10428.91 Da
• Isoelectric Point: 4.7163

>T228438 WP_256521052.1 NZ_CP089607:c110264-109962 [Rhodococcus qingshengii]
VRPIHDAQLGDLCPVLVLTRELVRPHLGTVTVAPIRAEGRGLSTELSLGEGNGVQDGSVVDCDAITTIAVSALGANRGFL
LPAQEALLSRALRSAFDLES

Nucleotide

• Length: 303 bp

>T228438 NZ_CP123953:c3885882-3885304 [Pseudomonas aeruginosa]
ATGTCCCTCGAACTGCTGAGCAACCGCCTCGAATTCCTCCGCGAAGCCGAACGCCTGAAAGACGTCCTGCGCTCCGCGCA
CACCTCCTCCGGCCGCCCGGAAAGCACCGCCGAACATAGCTGGCGGCTGTGCCTGATGGCACTGGCCTTCGAGGACCAAC
TGGCCGGCCTGGACCTCGGCAAGGTCCTGCGCATGTGCGTGGTCCACGACCTCGGCGAAGCGATCCACGGCGACATCCCC
GCCGTCGAGCAGGCCGCGCACCCGGACAAAGGCGAACAGGAACGCGCCGACCTGTTGCAGCTGACCCGTCACCTCGACGC
GCCGCTGCGCGACCGCCTGCTCGCCCTGTGGGACGAATACGAGCGCGGCGAGTCCGCCGAAGCCCTGGCAGTGAAGGCCC
TGGACAAGCTGGAAACCCTGCTCCAGCACACCCAGGGCGACAATCCGGCGGACTTCGACTATGCCTTCAACCTCGACTAC
GGCCGTCGCTATACCGACCGCGCACCGCTGTTCAAGACCCTGCGCGAGCTACTCGACGCCAGGACCCGACAGCGCCTCGC
AGCTCGGCGCGACGCCTAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7728.73 Da
• Isoelectric Point: 7.0125

>AT228438 WP_256521053.1 NZ_CP089607:c110480-110265 [Rhodococcus qingshengii]
MSTQIAVRLPEDIVAFLDSEVSGGRAKSRAQVVLRALEREQRRQLAVRDVEILSDGADSDVNIAAAHGKKN

Nucleotide

• Length: 216 bp

>AT228438 NZ_CP123953:c3886525-3885971 [Pseudomonas aeruginosa]
ATGACCGAGCCTCTCCCTTCTTCGTCCCTGGGCCCCGCCCTGCGTCGCTGGCGCCTGCTGCACCGGGTCAAGCAAACGCA
TGCCGCCGAATTGCTCGGCGTGGCGCAATCGACCATCTCGCGCTGGGAGTCCGGTACCCAGGCGCTGCTGGTGGAGGAAC
GGGCACGCCTGGAGCGTCTGCTCGGCGCCCGCCTGGAAGCCGCCGCCGACCAGGCACTGGCCCGCCTGGTCGAGGCCAAC
CCGCAACCCGTGCACCTGATCTGCGACCTCACCCATCGCCTGTTGGCCTGTTCGCCGGCACGCGCCGCGCAATTCGGCGT
GCCTCTCGGCGAACTGCTCGACCGTTCGCTGTGGCCCTATTGCAGCGAGGAAATCCTCCGCCAGGAAGCCACCCTCGAGG
AACTCGGCTGGCGCGAACTGCTGGCACCGCCGGCGCTGGAGTTCGCCAGCGGAGCGAACGCCTCGGCCATCGTGCCGATC
CGGCGCAGCCGTTGCCGCTGGACCCGCATGACCCTCTCGGACGGACGCGCGGTGCGCCTGGTGGAAACCCTCTGA