Detailed information of TA system TA228435

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafN(antitoxin)
• Location: 239499..240064
• Replicon: chromosome
• Accession: NZ_CP089603
• Organism: Vibrio furnissii strain VFN3

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: LUM42_RS01110
• Protein ID: WP_143683507.1
• Coordinates: 239777..240064 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U3BLE1
• Locus tag: LUM42_RS01105
• Protein ID: WP_000086647.1
• Coordinates: 239499..239780 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LUM42_RS01085 (LUM42_01085)	236347..236643	+	297	Protein_205	IS5/IS1182 family transposase	-
LUM42_RS01090 (LUM42_01090)	236702..237313	-	612	WP_014610887.1	mobile mystery protein B	-
LUM42_RS01095 (LUM42_01095)	237310..237798	-	489	WP_094147126.1	mobile mystery protein A	-
LUM42_RS01100 (LUM42_01100)	238630..239337	-	708	Protein_208	IS110 family transposase	-
LUM42_RS01105 (LUM42_01105)	239499..239780	+	282	WP_000086647.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LUM42_RS01110 (LUM42_01110)	239777..240064	+	288	WP_143683507.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LUM42_RS01115 (LUM42_01115)	240425..240610	-	186	Protein_211	IS21 family transposase	-
LUM42_RS01120 (LUM42_01120)	240789..241280	-	492	WP_232658366.1	AAA family ATPase	-
LUM42_RS01125 (LUM42_01125)	241689..242609	-	921	WP_232658367.1	IS5-like element ISVpa3 family transposase	-
LUM42_RS01130 (LUM42_01130)	242825..244144	+	1320	WP_232658368.1	MBL fold metallo-hydrolase	-
LUM42_RS01135 (LUM42_01135)	244220..244993	-	774	WP_154180621.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	bpsE	204924..242609	37685
	-	inside	IScluster/Tn	-	-	236503..242609	6106

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (9-74)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11009.65 Da
• Isoelectric Point: 5.1098

>T228435 WP_143683507.1 NZ_CP089603:239777-240064 [Vibrio furnissii]
MKVVWSPLALQKLSDAAEFISLDNPSAAEKWVNEVFDKTELLGSMPEMGRFVPEMPHTNYREIIFGHYRIIYSLSHEIRV
LTVRNCRQVLSEDDV

Nucleotide

• Length: 288 bp

>T228435 NZ_CP123953:2842124-2842423 [Pseudomonas aeruginosa]
ATGGTTGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10398.91 Da
• Isoelectric Point: 5.8650

>AT228435 WP_000086647.1 NZ_CP089603:239499-239780 [Vibrio furnissii]
MSRIHFDQDIQPLSEFRAGVASYIKQINETRRPLVITQRGKGVAVVLDVAEYEAMQEKIELLEEMRTAEAQLASGLGVSN
DDARAQVLGRIKK

Nucleotide

• Length: 282 bp

>AT228435 NZ_CP123953:2842420-2842710 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
CTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCGACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGCGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A4P8G0X0