228433

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 242103..242745 Replicon chromosome
Accession NZ_CP089601
Organism Bacillus cereus strain C-1

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag LRS65_RS01380 Protein ID WP_000635963.1
Coordinates 242395..242745 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag LRS65_RS01375 Protein ID WP_000004570.1
Coordinates 242103..242390 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRS65_RS01350 (LRS65_01350) 237420..238382 + 963 WP_000961166.1 UV DNA damage repair endonuclease UvsE -
LRS65_RS01355 (LRS65_01355) 238375..238947 - 573 WP_000908523.1 rhomboid family intramembrane serine protease -
LRS65_RS01360 (LRS65_01360) 239041..239400 + 360 WP_000583416.1 holo-ACP synthase -
LRS65_RS01365 (LRS65_01365) 239557..240507 + 951 WP_002004297.1 outer membrane lipoprotein carrier protein LolA -
LRS65_RS01370 (LRS65_01370) 240625..241794 + 1170 WP_000390615.1 alanine racemase -
LRS65_RS01375 (LRS65_01375) 242103..242390 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
LRS65_RS01380 (LRS65_01380) 242395..242745 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
LRS65_RS01385 (LRS65_01385) 242813..244981 + 2169 WP_000426242.1 Tex family protein -
LRS65_RS01390 (LRS65_01390) 245039..245155 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
LRS65_RS01395 (LRS65_01395) 245351..245809 + 459 WP_257115825.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T228433 WP_000635963.1 NZ_CP089601:242395-242745 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T228433 NZ_CP123953:625117-625299 [Pseudomonas aeruginosa]
ATGAAGTACAGCGAGTTTCGGCGCTGGTTGAAGGCTCGGGGAGTCATTTTCGAGCCTGCCAAGGGAAGCCACTTCAAGGT
TTACTACGGCGACAACCAGACGATTTTCCCCGACCATGGAGCCAAGGAAATAGGCGATGGGTTGAGGAAGAAGATAATCA
AAGACCTGGGCCTCAAGGATTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT228433 WP_000004570.1 NZ_CP089601:242103-242390 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT228433 NZ_CP123953:625332-625754 [Pseudomonas aeruginosa]
ATGTTTGACTATCCCGTAACGGTTCACGAGGAAGCCGGCAGCGTGTGGGTCAGCTGCGACGATGTACCTGAAATGGCGAG
CGCCGGAGATACCGTTGATGAAGCGCTGCTGGACGCTGTGGAAGGCCTAGAGTCGGCCCTGTCGCTCTATGTGGACCGGC
GGCAGTCGATCCCGCTGCCGTCGAAAGGCAAAGCGGGTCAGTCGATCGTTCGATTGCCCGCGCTCACTTCGGCCAAGATT
GCGCTGTGGAACACCATGCTGGCTCAGAACGTAGGGAAGGCGGAACTGGCTCGCCGGCTGGGAGTGAATCGCGTTCAGGT
AGACCGCCTGGTGGATCTGCTCCACGGGTCGAAGATCGAAGCGGTGGAACATGCTCTGGCGATCTTGGGCCAACGGTTGG
CTGTGACTGTCATTGCGGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References