Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 156271..156941 Replicon plasmid pKP29499-1
Accession NZ_CP089599
Organism Klebsiella pneumoniae strain KP29499

Toxin (Protein)


Gene name vagD Uniprot ID Q6U619
Locus tag LVE73_RS27405 Protein ID WP_004213072.1
Coordinates 156271..156714 (-) Length 148 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q6U620
Locus tag LVE73_RS27410 Protein ID WP_004213073.1
Coordinates 156711..156941 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVE73_RS27370 (LVE73_27370) 151681..151956 + 276 WP_004213932.1 carboxymuconolactone decarboxylase family protein -
LVE73_RS27375 (LVE73_27375) 152019..152510 + 492 WP_011251327.1 DM13 domain-containing protein -
LVE73_RS27380 (LVE73_27380) 152559..153479 + 921 WP_232700329.1 DUF1471 domain-containing protein -
LVE73_RS27385 (LVE73_27385) 153570..153973 + 404 Protein_167 GAF domain-containing protein -
LVE73_RS27390 (LVE73_27390) 154491..155127 - 637 Protein_168 mucoid phenotype regulator RmpA2 -
LVE73_RS27395 (LVE73_27395) 155544..155848 + 305 Protein_169 transposase -
LVE73_RS27400 (LVE73_27400) 155871..156122 - 252 WP_186987481.1 hypothetical protein -
LVE73_RS27405 (LVE73_27405) 156271..156714 - 444 WP_004213072.1 type II toxin-antitoxin system VapC family toxin Toxin
LVE73_RS27410 (LVE73_27410) 156711..156941 - 231 WP_004213073.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LVE73_RS27415 (LVE73_27415) 157549..158682 + 1134 WP_004213075.1 DUF3800 domain-containing protein -
LVE73_RS27420 (LVE73_27420) 158698..158991 + 294 WP_004213076.1 hypothetical protein -
LVE73_RS27425 (LVE73_27425) 158981..159187 - 207 WP_004213077.1 hypothetical protein -
LVE73_RS27430 (LVE73_27430) 159539..159829 + 291 WP_004213078.1 hypothetical protein -
LVE73_RS27435 (LVE73_27435) 159819..160718 + 900 WP_004225022.1 nucleotide-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2 1..229581 229581


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 148 a.a.        Molecular weight: 16006.74 Da        Isoelectric Point: 9.3642

>T228430 WP_004213072.1 NZ_CP089599:c156714-156271 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Download         Length: 444 bp

>T228430 NZ_CP123951:3789611-3789718 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8677.73 Da        Isoelectric Point: 4.4563

>AT228430 WP_004213073.1 NZ_CP089599:c156941-156711 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT228430 NZ_CP123951:c3789564-3789498 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q6U619

Antitoxin

Source ID Structure
AlphaFold DB Q6U620

References