Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5439605..5440230 Replicon chromosome
Accession NZ_CP089598
Organism Klebsiella pneumoniae strain KP29499

Toxin (Protein)


Gene name vapC Uniprot ID A0A330XB05
Locus tag LVE73_RS26195 Protein ID WP_012737091.1
Coordinates 5439605..5439988 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LVE73_RS26200 Protein ID WP_004150355.1
Coordinates 5439988..5440230 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVE73_RS26180 (5436971) 5436971..5437873 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LVE73_RS26185 (5437870) 5437870..5438505 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LVE73_RS26190 (5438502) 5438502..5439431 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LVE73_RS26195 (5439605) 5439605..5439988 - 384 WP_012737091.1 type II toxin-antitoxin system VapC family toxin Toxin
LVE73_RS26200 (5439988) 5439988..5440230 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LVE73_RS26205 (5440435) 5440435..5441352 + 918 WP_012737090.1 alpha/beta hydrolase -
LVE73_RS26210 (5441366) 5441366..5442307 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LVE73_RS26215 (5442352) 5442352..5442789 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LVE73_RS26220 (5442786) 5442786..5443646 - 861 WP_004146232.1 virulence factor BrkB family protein -
LVE73_RS26225 (5443640) 5443640..5444239 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14266.53 Da        Isoelectric Point: 7.2771

>T228427 WP_012737091.1 NZ_CP089598:c5439988-5439605 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRCEAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKGFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T228427 NZ_CP123951:3541231-3541518 [Escherichia coli]
ATGACTTATACAGTAAAATTCCGGGATGATGCACTGAAAGAATGGCTCAGACTGGACAAGACCATTCAGCAGCAGTTCGC
AAAAAAACTGAAAAAATGCAGTGAAAATCCTCATATAGCTTCTGCAAAACTACGTGGATTAAAGGACTGCTATAAAATCA
AACTTCGCGCATCAGGTTTTCGTCTGGTTTATCAGGTTATTGATGATATGTTAATTATTGCGGTCGTCGCAGTAGGTAAA
CGTGAACGCAGCAACGTTTATCATCTCGCCAGTGAACGGATGAAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT228427 WP_004150355.1 NZ_CP089598:c5440230-5439988 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT228427 NZ_CP123951:3540983-3541234 [Escherichia coli]
ATGCCCAGTATCATTTTGTCTGACACCAGTGCAAGCGTCAGTGAGCTGAAAAAAAATCCGATGGCAACTGTCAGTGCCGG
AGAGGGGTATCCTGTTGCCATTCTTAACCGGAATCAGCCAGCATTCTACTGTGTCCCTGCTGAGCTCTATGAAAGAATGC
TTGATGCCTTGGATGACCAGGAGCTGGTAAAACTGGTTACTGAACGCAGCAGTCAGCCTCTGCATGATGTGGACCTGGAC
AGTTATTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A330XB05

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References