Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4802366..4803176 Replicon chromosome
Accession NZ_CP089598
Organism Klebsiella pneumoniae strain KP29499

Toxin (Protein)


Gene name KacT Uniprot ID A0A332NV98
Locus tag LVE73_RS23220 Protein ID WP_014908042.1
Coordinates 4802366..4802899 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag LVE73_RS23225 Protein ID WP_002887278.1
Coordinates 4802910..4803176 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVE73_RS23215 (4801197) 4801197..4802318 + 1122 WP_012737335.1 cupin domain-containing protein -
LVE73_RS23220 (4802366) 4802366..4802899 - 534 WP_014908042.1 type II toxin-antitoxin system toxin KacT Toxin
LVE73_RS23225 (4802910) 4802910..4803176 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
LVE73_RS23230 (4803279) 4803279..4804712 - 1434 WP_014908043.1 Cu(+)/Ag(+) sensor histidine kinase -
LVE73_RS23235 (4804702) 4804702..4805385 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
LVE73_RS23240 (4805557) 4805557..4806942 + 1386 WP_012737332.1 efflux transporter outer membrane subunit -
LVE73_RS23245 (4806960) 4806960..4807304 + 345 WP_012737331.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(87-150)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19795.68 Da        Isoelectric Point: 5.2614

>T228425 WP_014908042.1 NZ_CP089598:c4802899-4802366 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGLGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T228425 NZ_CP123951:3160537-3160640 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT228425 WP_002887278.1 NZ_CP089598:c4803176-4802910 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT228425 NZ_CP123951:c3160678-3160407 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A332NV98

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References