Detailed information of TA system TA022841

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 6527031..6527640
• Replicon: chromosome
• Accession: NC_010628
• Organism: Nostoc punctiforme PCC 73102 (Nostoc punctiforme ATCC 29133)

Toxin (Protein)

• Gene name: relE
• Uniprot ID: B2J4A8
• Locus tag: NPUN_RS26695
• Protein ID: WP_012411550.1
• Coordinates: 6527031..6527408 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: NPUN_RS26700
• Protein ID: WP_012411551.1
• Coordinates: 6527401..6527640 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NPUN_RS26675	6523047..6523901	-	855	WP_012411547.1	sulfurtransferase	-
NPUN_RS26680	6523924..6524367	-	444	WP_041565648.1	hypothetical protein	-
NPUN_RS26685	6524481..6525146	+	666	WP_012411549.1	LuxR family transcriptional regulator	-
NPUN_RS26690	6525313..6526855	+	1543	Protein_5337	IS5 family transposase	-
NPUN_RS26695	6527031..6527408	-	378	WP_012411550.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
NPUN_RS26700	6527401..6527640	-	240	WP_012411551.1	hypothetical protein	Antitoxin
NPUN_RS26705	6527784..6528365	-	582	WP_012411552.1	Uma2 family endonuclease	-
NPUN_RS37890	6528362..6529144	-	783	WP_167315663.1	restriction endonuclease subunit S	-
NPUN_RS37895	6529292..6530359	-	1068	WP_052304657.1	N-6 DNA methylase	-
NPUN_RS26715	6530429..6531322	+	894	WP_012406904.1	transposase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	6525406..6526512	1106

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (23-108)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14212.10 Da
• Isoelectric Point: 6.3726

>T22841 WP_012411550.1 NC_010628:c6527408-6527031 [Nostoc punctiforme PCC 73102]
MTDQPFIQVEASATFNRNLRTLAKKYRSIRNDIQPVIEQLQQGELPGDQIPGVSYAIFKLRVRNSDAQKGKSGGYRLIYY
VKTATGIILLTVYTKSEQVDIAAKDIQSIIAEYEQQTINDEERDI

Nucleotide

• Length: 378 bp

>T22841 NC_010628:c6527408-6527031 [Nostoc punctiforme PCC 73102]
ATGACTGATCAGCCTTTTATTCAAGTTGAGGCTTCTGCAACCTTCAACCGAAATCTACGCACTCTTGCTAAGAAATATCG
CAGTATTCGGAATGATATACAACCTGTTATCGAACAACTTCAGCAAGGAGAGTTGCCAGGAGATCAAATACCTGGTGTTA
GTTATGCAATCTTCAAGTTGAGAGTCCGAAATAGCGACGCTCAAAAAGGTAAAAGTGGTGGCTATCGTCTTATTTATTAC
GTCAAAACAGCAACTGGAATCATTCTGCTTACTGTTTATACCAAATCAGAACAAGTTGATATTGCAGCAAAGGATATTCA
GAGCATCATTGCAGAATATGAGCAACAGACAATCAATGATGAAGAAAGGGATATATAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8893.02 Da
• Isoelectric Point: 3.9367

>AT22841 WP_012411551.1 NC_010628:c6527640-6527401 [Nostoc punctiforme PCC 73102]
MKALKVMATINEEGQLTLDHPLTIDKNSRVEVIVLIPDEETPDETSQTEVLADFRQAWQEAMTGQTIPVAQLWEGLKDD

Nucleotide

• Length: 240 bp

>AT22841 NC_010628:c6527640-6527401 [Nostoc punctiforme PCC 73102]
ATGAAAGCACTTAAAGTCATGGCAACGATCAATGAGGAGGGACAACTAACCTTAGATCATCCTCTCACAATAGATAAAAA
TAGCCGTGTAGAAGTCATTGTTTTAATTCCAGATGAAGAAACCCCAGATGAGACATCTCAGACAGAAGTTTTAGCAGATT
TTCGGCAAGCTTGGCAGGAAGCCATGACTGGACAAACAATTCCCGTTGCTCAACTTTGGGAAGGTCTTAAAGATGACTGA

Structures

Toxin

• AlphaFold DB: B2J4A8