Detailed information of TA system TA022840

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicA
• Location: 5031243..5031661
• Replicon: chromosome
• Accession: NC_010628
• Organism: Nostoc punctiforme PCC 73102 (Nostoc punctiforme ATCC 29133)

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: NPUN_RS20225
• Protein ID: WP_012410353.1
• Coordinates: 5031243..5031425 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: B2J6I2
• Locus tag: NPUN_RS20230
• Protein ID: WP_012410354.1
• Coordinates: 5031437..5031661 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NPUN_RS20210	5027056..5028381	+	1326	WP_012410350.1	hypothetical protein	-
NPUN_RS20215	5028498..5029055	-	558	WP_012410351.1	exonuclease	-
NPUN_RS20220	5029293..5031047	-	1755	WP_012410352.1	arginine--tRNA ligase	-
NPUN_RS20225	5031243..5031425	+	183	WP_012410353.1	type II toxin-antitoxin system HicA family toxin	Toxin
NPUN_RS20230	5031437..5031661	+	225	WP_012410354.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
NPUN_RS20235	5031796..5033295	-	1500	WP_012410355.1	UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase	-
NPUN_RS20240	5033381..5035534	-	2154	WP_012410356.1	glycine--tRNA ligase subunit beta	-
NPUN_RS20245	5035809..5036426	+	618	WP_148220345.1	PEP-CTERM sorting domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4916714..5102794	186080

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-60)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-72)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6836.70 Da
• Isoelectric Point: 7.2014

>T22840 WP_012410353.1 NC_010628:5031243-5031425 [Nostoc punctiforme PCC 73102]
MKVRDAIERVEADGWYLDRTKDSHQQFKHPDKPGLVTVPGKFSDDLAPGTLSSIWRQAQL

Nucleotide

• Length: 183 bp

>T22840 NC_010628:5031243-5031425 [Nostoc punctiforme PCC 73102]
ATGAAAGTACGTGATGCGATCGAGCGAGTGGAAGCTGATGGTTGGTATCTTGATAGAACTAAAGATAGTCATCAACAGTT
CAAGCATCCTGATAAACCAGGTTTAGTTACAGTTCCAGGTAAATTCTCTGACGACTTAGCCCCTGGTACTCTCAGTAGTA
TTTGGAGGCAAGCACAATTATAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8092.21 Da
• Isoelectric Point: 4.2243

>AT22840 WP_012410354.1 NC_010628:5031437-5031661 [Nostoc punctiforme PCC 73102]
MHYVVVIEKADSNYSAYVPDLPGCVTTGATLEEVKQMIAEAIEFHIEGMLEDGLPIPKPTSIAHEVEVANYSKT

Nucleotide

• Length: 225 bp

>AT22840 NC_010628:5031437-5031661 [Nostoc punctiforme PCC 73102]
ATGCATTACGTAGTGGTGATTGAAAAGGCGGATAGTAATTACTCTGCTTATGTGCCTGATTTACCAGGTTGTGTAACTAC
AGGTGCAACCTTAGAAGAAGTCAAGCAGATGATTGCAGAAGCTATTGAATTTCATATAGAAGGAATGCTAGAAGATGGTT
TACCTATTCCTAAACCTACAAGCATCGCTCATGAGGTTGAAGTTGCAAACTACAGCAAAACATAA

Structures

Antitoxin

• AlphaFold DB: B2J6I2