Detailed information of TA system TA022839

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2835061..2835610
• Replicon: chromosome
• Accession: NC_010628
• Organism: Nostoc punctiforme PCC 73102 (Nostoc punctiforme ATCC 29133)

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: B2J7E4
• Locus tag: NPUN_RS11515
• Protein ID: WP_012408876.1
• Coordinates: 2835061..2835399 (-)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: B2J7E5
• Locus tag: NPUN_RS11520
• Protein ID: WP_012408877.1
• Coordinates: 2835386..2835610 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NPUN_RS11490	2830469..2831314	+	846	WP_012408872.1	SDR family oxidoreductase	-
NPUN_RS40050	2831455..2832622	-	1168	Protein_2305	IS630 family transposase	-
NPUN_RS38885	2832746..2832928	-	183	WP_083782382.1	hypothetical protein	-
NPUN_RS11505	2833522..2834673	-	1152	WP_012408874.1	AI-2E family transporter	-
NPUN_RS11510	2834764..2834955	-	192	WP_041566096.1	hypothetical protein	-
NPUN_RS11515	2835061..2835399	-	339	WP_012408876.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
NPUN_RS11520	2835386..2835610	-	225	WP_012408877.1	CopG family transcriptional regulator	Antitoxin
NPUN_RS37520	2835898..2836545	+	648	WP_012408878.1	hypothetical protein	-
NPUN_RS38890	2836697..2836912	-	216	Protein_2312	DUF1016 domain-containing protein	-
NPUN_RS11535	2836990..2837499	-	510	WP_012408879.1	hypothetical protein	-
NPUN_RS11540	2837529..2838443	-	915	WP_012408880.1	homoserine kinase	-
NPUN_RS11545	2838617..2839357	+	741	WP_012408881.1	RNA methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-108)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12344.24 Da
• Isoelectric Point: 9.5902

>T22839 WP_012408876.1 NC_010628:c2835399-2835061 [Nostoc punctiforme PCC 73102]
MKRGDIYYANLSPAVGSEMDKRRPVLIVSNDANNRAASTVTILPLTSNVSRVYPFEVLLNPENSGLPLASKVQAQQVRTI
SKRRIVSEVVNSLNEELMQLVDAALKLHLGLE

Nucleotide

• Length: 339 bp

>T22839 NC_010628:c2835399-2835061 [Nostoc punctiforme PCC 73102]
ATGAAGCGTGGTGATATTTATTACGCTAATCTCAGTCCGGCTGTAGGCTCTGAGATGGATAAACGCCGCCCAGTGCTGAT
AGTCAGTAATGATGCCAATAATCGTGCAGCTAGCACGGTAACAATTTTACCGCTTACATCCAATGTTAGCCGTGTTTACC
CGTTCGAGGTTTTACTGAATCCAGAGAATAGTGGTTTACCATTAGCTTCCAAAGTACAGGCGCAGCAGGTACGAACAATT
TCTAAGCGAAGGATTGTGTCAGAGGTGGTAAATAGTTTAAATGAAGAGTTGATGCAATTAGTAGATGCTGCTCTCAAACT
CCATTTAGGGTTGGAGTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8440.45 Da
• Isoelectric Point: 4.1191

>AT22839 WP_012408877.1 NC_010628:c2835610-2835386 [Nostoc punctiforme PCC 73102]
MQVEKLSISLPASLVQFIEGYKVAHDCKSRSQVIEKALQLLRTQELEEAYREASAEVENDWDITITDGLTDEAW

Nucleotide

• Length: 225 bp

>AT22839 NC_010628:c2835610-2835386 [Nostoc punctiforme PCC 73102]
ATGCAAGTTGAAAAATTATCAATTTCTTTACCAGCGTCCCTGGTGCAATTTATTGAAGGTTACAAAGTAGCTCATGACTG
TAAATCTCGCTCCCAAGTCATTGAAAAGGCATTGCAGTTGTTGCGAACACAGGAACTAGAAGAAGCTTACCGAGAAGCAT
CTGCTGAAGTTGAGAATGATTGGGATATCACGATTACAGATGGATTAACCGATGAAGCGTGGTGA

Structures

Toxin

• AlphaFold DB: B2J7E4

Antitoxin

• AlphaFold DB: B2J7E5