Detailed information of TA system TA228386

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2706710..2707239
• Replicon: chromosome
• Accession: NZ_CP089586
• Organism: Staphylococcus aureus strain E1185_IV_ST12

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: LUU82_RS13475
• Protein ID: WP_000621175.1
• Coordinates: 2706877..2707239 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: LUU82_RS13470
• Protein ID: WP_000948331.1
• Coordinates: 2706710..2706880 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LUU82_RS13440 (2701738)	2701738..2702298	+	561	WP_001092417.1	K(+)-transporting ATPase subunit C	-
LUU82_RS13445 (2702515)	2702515..2702994	+	480	WP_001287085.1	hypothetical protein	-
LUU82_RS13450 (2702987)	2702987..2704570	+	1584	WP_001294627.1	PH domain-containing protein	-
LUU82_RS13455 (2704557)	2704557..2705048	+	492	WP_001205910.1	PH domain-containing protein	-
LUU82_RS13460 (2705052)	2705052..2705411	+	360	WP_000581197.1	holo-ACP synthase	-
LUU82_RS13465 (2705477)	2705477..2706625	+	1149	WP_001281154.1	alanine racemase	-
LUU82_RS13470 (2706710)	2706710..2706880	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
LUU82_RS13475 (2706877)	2706877..2707239	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LUU82_RS13480 (2707588)	2707588..2708589	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
LUU82_RS13485 (2708708)	2708708..2709034	+	327	WP_001052491.1	anti-sigma factor antagonist	-
LUU82_RS13490 (2709036)	2709036..2709515	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
LUU82_RS13495 (2709490)	2709490..2710260	+	771	WP_001041102.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T228386 WP_000621175.1 NZ_CP089586:2706877-2707239 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T228386 NZ_CP123897:c3331954-3331655 [Methylomonas sp. UP202]
ATGAAATACGAATTGCAAAGCACGCAGACATTTGCCGAATGGTTCTCCAGGCAAAAGGACAAGACGGTTAAAAACCAGTT
GCTGTCCAGGCTGGCACGGATTGAGAACGGCAATTTCGGCGACAGCAAAACCTTGTCCGCAAATTTGTTTGAGCTACGCT
GCTTTTTTGGTGGCGGCATTAGGTTGTATTACACAATCCGTAATCAACGAATTGTTTTATTGCTAGCTGGTGGCGACAAA
TCCAGCCAAAACAAAGACATTGAAAAAGCCAAAGCTATATTGAATTCCTTGGAGGATTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT228386 WP_000948331.1 NZ_CP089586:2706710-2706880 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT228386 NZ_CP123897:c3331651-3331373 [Methylomonas sp. UP202]
ATGCCTGAAACTATTACCGCTTTTGATATTGCCGAACATTTGGAAACAGAAGCCGATGTCCGTGAATTTTTGCGGGAAAC
GGCAAGCAACGGCAGCCCTTCCGATTTTATACACGCACTTAATATTGCCGCTCGCGCCAAAGGTATGAGCGAAGTTGCCA
AGCAAGCTGGGGTTACTAGAGCCAGCCTTTACAAATCCTTGGCGGAAGGCGGTAACCCCCGATTTGAAACCGTTGCCAAA
ATTGTCGAAGCATTAGGTTGTAAATTGATTGTCTCCTAG

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1