Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 2686959..2687473 Replicon chromosome
Accession NZ_CP089586
Organism Staphylococcus aureus strain E1185_IV_ST12

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag LUU82_RS13385 Protein ID WP_000581792.1
Coordinates 2687264..2687473 (+) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag LUU82_RS13380 Protein ID WP_000138932.1
Coordinates 2686959..2687252 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LUU82_RS13360 (2682430) 2682430..2683071 + 642 WP_000483153.1 thiamine phosphate synthase -
LUU82_RS13365 (2683157) 2683157..2684029 + 873 WP_000725802.1 membrane protein insertase YidC -
LUU82_RS13370 (2684634) 2684634..2685281 - 648 WP_001187630.1 HD domain-containing protein -
LUU82_RS13375 (2685309) 2685309..2686793 - 1485 WP_000571549.1 cardiolipin synthase -
LUU82_RS13380 (2686959) 2686959..2687252 + 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
LUU82_RS13385 (2687264) 2687264..2687473 + 210 WP_000581792.1 heavy metal-associated domain-containing protein Toxin
LUU82_RS13390 (2687601) 2687601..2687738 + 138 WP_000828354.1 Lmo0850 family protein -
LUU82_RS13395 (2688002) 2688002..2689204 - 1203 WP_001109942.1 FtsW/RodA/SpoVE family cell cycle protein -
LUU82_RS13400 (2689522) 2689522..2690592 + 1071 WP_000159631.1 D-alanine--D-alanine ligase -
LUU82_RS13405 (2690607) 2690607..2691965 + 1359 WP_000611465.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T228385 WP_000581792.1 NZ_CP089586:2687264-2687473 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T228385 NZ_CP123897:c3331651-3331373 [Methylomonas sp. UP202]
ATGCCTGAAACTATTACCGCTTTTGATATTGCCGAACATTTGGAAACAGAAGCCGATGTCCGTGAATTTTTGCGGGAAAC
GGCAAGCAACGGCAGCCCTTCCGATTTTATACACGCACTTAATATTGCCGCTCGCGCCAAAGGTATGAGCGAAGTTGCCA
AGCAAGCTGGGGTTACTAGAGCCAGCCTTTACAAATCCTTGGCGGAAGGCGGTAACCCCCGATTTGAAACCGTTGCCAAA
ATTGTCGAAGCATTAGGTTGTAAATTGATTGTCTCCTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT228385 WP_000138932.1 NZ_CP089586:2686959-2687252 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT228385 NZ_CP123897:c3331954-3331655 [Methylomonas sp. UP202]
ATGAAATACGAATTGCAAAGCACGCAGACATTTGCCGAATGGTTCTCCAGGCAAAAGGACAAGACGGTTAAAAACCAGTT
GCTGTCCAGGCTGGCACGGATTGAGAACGGCAATTTCGGCGACAGCAAAACCTTGTCCGCAAATTTGTTTGAGCTACGCT
GCTTTTTTGGTGGCGGCATTAGGTTGTATTACACAATCCGTAATCAACGAATTGTTTTATTGCTAGCTGGTGGCGACAAA
TCCAGCCAAAACAAAGACATTGAAAAAGCCAAAGCTATATTGAATTCCTTGGAGGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References